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Free keywords:
Affinity Labels/*chemistry Aptamers, Nucleotide/*chemistry Cell Line, Tumor Humans Proteins/*chemistry/metabolism Proto-Oncogene Proteins c-met/chemistry/metabolism SELEX Aptamer Technique Ultraviolet Rays
Abstract:
A most able label: Labeled aptamers can be cross-linked to their target structures in a light-dependent and highly specific manner as a result of a new strategy termed aptamer-based affinity labeling (ABAL) of proteins. The aptamer-protein complexes can be enriched in vitro, from a cellular lysate and from the surface of living cells, opening new ways to study aptamer interactions in biological contexts.