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  Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo

Mittmann, W., Wallace, D. J., Czubayko, U., Herb, J. T., Schaefer, A. T., Looger, L. L., et al. (2011). Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo. Nature Neuroscience, 14(8), 1089-93. doi:10.1038/nn.2879.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-64A2-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0006-6A16-B
Genre: Journal Article

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 Creators:
Mittmann, W.1, Author
Wallace, D. J.2, Author
Czubayko, U.1, Author
Herb, J. T.1, Author
Schaefer, A. T.1, Author
Looger, L. L.1, Author
Denk, W.1, Author
Kerr, J. N.2, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society, Ludwig-Erhard-Allee 2, 53175 Bonn, DE, ou_2173678              

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Free keywords: Animals Animals, Newborn Axons/metabolism Calcium/*metabolism Dendrites/metabolism Evoked Potentials, Somatosensory/*physiology Green Fluorescent Proteins/genetics Mice Mice, Inbred C57BL Mice, Transgenic Nerve Net/physiology Neurons/cytology/*physiology Photons/diagnostic use Physical Stimulation/methods Probability Reaction Time Somatosensory Cortex/*cytology Statistics as Topic
 Abstract: Multiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found that this approach could be used to record and quantify spontaneous and sensory-evoked activity in populations of L5 neuronal somata located as much as 800 mum below the pia. In addition, we found that RAMM could be used to simultaneously image activity from large (80) populations of apical dendrites and follow these dendrites down to their somata of origin.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Issued
 Pages: -
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 Rev. Type: Peer
 Identifiers: Other: 21743473
DOI: 10.1038/nn.2879
ISSN: 1546-1726 (Electronic)
ISSN: 1097-6256 (Linking)
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Title: Nature Neuroscience
  Alternative Title : Nat. Neurosci.
Source Genre: Journal
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Pages: - Volume / Issue: 14 (8) Sequence Number: - Start / End Page: 1089 - 93 Identifier: -