English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 DetailsSummary
  Liquid application method for time-resolved analyses by serial synchrotron crystallography

Mehrabi, P., Schulz, E.-C., Agthe, M., Horrell, S., Bourenkov, G., von Stetten, D., et al. (2019). Liquid application method for time-resolved analyses by serial synchrotron crystallography. Nature methods, 16(10), 979-982. doi:10.1038/s41592-019-0553-1.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0004-ADB5-D Version Permalink: https://hdl.handle.net/21.11116/0000-0004-D998-C
Genre: Journal Article

Files

show Files
hide Files
:
s41592-019-0553-1.pdf (Publisher version), 4MB
 
File Permalink:
-
Name:
s41592-019-0553-1.pdf
Description:
-
OA-Status:
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
41592_2019_553_MOESM1_ESM.pdf (Supplementary material), 2MB
 
File Permalink:
-
Name:
41592_2019_553_MOESM1_ESM.pdf
Description:
Supplementary Figures 1–5, Supplementary Note, Supplementary Tables 1–3
OA-Status:
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://dx.doi.org/10.1038/s41592-019-0553-1 (Publisher version)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Mehrabi, P.1, Author           
Schulz, E.-C.1, 2, Author           
Agthe, M.3, Author
Horrell, S.3, Author
Bourenkov, G.4, Author
von Stetten, D.4, Author
Leimkohl, J.-P.5, Author           
Schikora, H.5, Author           
Schneider, T. R.4, Author
Pearson, A. R.2, 3, Author
Tellkamp, F.5, Author           
Miller, R. J. D.1, 2, 6, Author           
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Centre for Ultrafast Imaging, Universität Hamburg, ou_persistent22              
3Institute for Nanostructure and Solid State Physics, Universität Hamburg, ou_persistent22              
4European Molecular Biology Laboratory, Hamburg Unit c/o Deutsches Elektronen Synchrotron, ou_persistent22              
5Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
6University of Toronto, Departments of Chemistry and Physics, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

Details

show
hide
Language(s): eng - English
 Dates: 2019-02-112019-08-062019-09-162019
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-019-0553-1
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : The authors thank D. Oberthuer (CFEL, DESY Hamburg) for the provision of the GlcNAc3 substrate immediately before data collection. The LAMA TR-SSX data were collected at beamline P14 and P14-2 (T-REXX) operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Construction of T-REXX was funded by the BMBF (Verbund Forschungsprojekt 05K16GU1). The authors are grateful to R. Dousza, L. Bultema and J. Besaw for their technical assistance during data collection. T-REXX beamtime was awarded as part of the EMBL BAG MX-660. The authors gratefully acknowledge the support provided by the Max Planck Society and the excellence cluster ‘The Hamburg Centre for Ultrafast Imaging—Structure, Dynamics and Control of Matter at the Atomic Scale’ of the Deutsche Forschungsgemeinschaft EXC 1074 project ID 194651731 (R.J.D.M.). P.M. was supported by the Alexander von Humboldt-Stiftung for postdoctoral researchers.
Grant ID : -
Funding program : -
Funding organization : -

Source 1

show
hide
Title: Nature methods
  Other : Nature methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Pub. Group
Pages: 4 Volume / Issue: 16 (10) Sequence Number: - Start / End Page: 979 - 982 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556