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Influenza virus Vaccine production Suspension MDCK Chemically defined medium Wave bioreactor
Abstract:
An adherently growing MDCK cell line was adapted in a two-step process in a fully defined medium
and in suspension. The resulting MDCK.SUS2 cells were subsequently evaluated for their potential as
host cells for influenza vaccine production in two lab-scale bioreactors (wave and stirred-tank). Cell
concentrations up to 2.3×106 cells/mL were obtained after 96 h, which is slightly higher than cell concentrations
obtained with adherent MDCK cells cultivated on microcarriers (2 g/L). Infections with influenza
A/PR/8/34 and B/Malaysia resulted in high virus titers (2.90 and 2.75 logHAunits/100uL, respectively).
The monitoring of extracellular metabolites, including amino acids, revealed a change in some of the
metabolite consumption or release profiles, which indicates changes in metabolism during the adaptation
process. Overall, the MDCK.SUS2 cell line represents a new cell substrate for a robust influenza
vaccine production in a fully defined process.
© 2010 Elsevier Ltd. All rights reserved.
[accessed September 21st, 2010]
