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  High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles.

Fricke, P., Mance, D., Chevelkov, V., Giller, K., Becker, S., Baldus, M., et al. (2016). High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles. Journal of Biomolecular NMR, 65(3-4), 121-126. doi:10.1007/s10858-016-0044-y.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-B05B-0 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-ABEE-4
Genre: Journal Article

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 Creators:
Fricke, P., Author
Mance, D. , Author
Chevelkov, V., Author
Giller, K.1, Author           
Becker, S.1, Author           
Baldus, M., Author
Lange, A., Author
Affiliations:
1Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: Proteins; Type III Secretion System; Solid-State NMR; DNP; Dynamics; PRE
 Abstract: The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800 MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22 %, indicating that inhomogeneous broadening is not the dominant effect that limits the resolution of T3SS needles under DNP conditions. The outstanding spectral resolution of this system under DNP conditions can be attributed to its low overall flexibility.

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Language(s): eng - English
 Dates: 2016-06-282016-08
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s10858-016-0044-y
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 65 (3-4) Sequence Number: - Start / End Page: 121 - 126 Identifier: -