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  Longitudinal 19F magnetic resonance imaging of brain oxygenation in a mouse model of vascular cognitive impairment using a cryogenic radiofrequency coil

Khalil, A., Mueller, S., Foddis, M., Mosch, L., Lips, J., Przesdzing, I., et al. (2019). Longitudinal 19F magnetic resonance imaging of brain oxygenation in a mouse model of vascular cognitive impairment using a cryogenic radiofrequency coil. Magnetic Resonance Materials in Physics, Biology and Medicine, 32(1), 105-144. doi:10.1007/s10334-018-0712-x.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0002-87F0-6 Versions-Permalink: https://hdl.handle.net/21.11116/0000-0003-886E-9
Genre: Zeitschriftenartikel

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Urheber

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 Urheber:
Khalil, Ahmed1, 2, 3, Autor           
Mueller, Susanne 1, 4, Autor
Foddis, Marco 1, Autor
Mosch, Larissa 1, Autor
Lips, Janet 1, Autor
Przesdzing, Ingo 1, Autor
Temme, Sebastian 5, Autor
Flögel, Ulrich 5, Autor
Dirnagl, Ulrich 1, 4, 6, Autor
Boehm-Sturm, Philipp 1, 4, Autor
Affiliations:
1Center for Stroke Research, Charité University Medicine Berlin, Germany, ou_persistent22              
2Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, Leipzig, DE, ou_634549              
3Berlin School of Mind and Brain, Humboldt University Berlin, Germany, ou_persistent22              
4NeuroCure Cluster of Excellence, Charité University Medicine Berlin, Germany, ou_persistent22              
5Department of Molecular Cardiology, University Hospital Düsseldorf, Germany, ou_persistent22              
6Berlin Institute of Health (BIH), Germany, ou_persistent22              

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Schlagwörter: Oxygenation; Vascular cognitive impairment; 19F-MRI; Perfluoro-15-crown-5-ether
 Zusammenfassung: INTRODUCTION:
We explored the use of a perfluoro-15-crown-5 ether nanoemulsion (PFC) for measuring tissue oxygenation using a mouse model of vascular cognitive impairment.
METHODS:
Seventeen C57BL/6 mice underwent stereotactic injection of PFC coupled to a fluorophore into the striatum and corpus callosum. Combined 1H/19F magnetic resonance imaging (MRI) to localize the PFC and R1 mapping to assess pO2 were performed. The effect of gas challenges on measured R1 was investigated. All mice then underwent bilateral implantation of microcoils around the common carotid arteries to induce global cerebral hypoperfusion. 19F-MRI and R1 mapping were performed 1 day, 1 week, and 4 weeks after microcoil implantation. In vivo R1 values were converted to pO2 through in vitro calibration. Tissue reaction to the PFC was assessed through ex vivo immunohistochemistry of microglial infiltration.
RESULTS:
R1 increased with increasing oxygen concentrations both in vitro and in vivo and the strength of the 19F signal remained largely stable over 4 weeks. In the two mice that received all four scans, tissue pO2 decreased after microcoil implantation and recovered 4 weeks later. We observed infiltration of the PFC deposits by microglia.
DISCUSSION:
Despite remaining technical challenges, intracerebrally injected PFC is suitable for monitoring brain oxygenation in vivo

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Sprache(n): eng - English
 Datum: 2018-10-142018-06-292018-10-222018-11-122019-02
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1007/s10334-018-0712-x
PMID: 30421249
Anderer: Epub ahead of print
 Art des Abschluß: -

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Projektinformation

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Projektname : -
Grant ID : BIH_PRO_317
Förderprogramm : -
Förderorganisation : Stiftung Charité
Projektname : NeuroCure - neue Perspektiven in der Therapie neurologischer Erkrankungen / EXC 257
Grant ID : -
Förderprogramm : -
Förderorganisation : Deutsche Forschungsgemeinschaft (DFG)
Projektname : Master switches bei kardialer Ischämie / SFB 1116
Grant ID : -
Förderprogramm : -
Förderorganisation : Deutsche Forschungsgemeinschaft (DFG)
Projektname : -
Grant ID : FL 303/6-1/
Förderprogramm : -
Förderorganisation : Deutsche Forschungsgemeinschaft (DFG)
Projektname : -
Grant ID : 01EO0801
Förderprogramm : -
Förderorganisation : German Federal Ministry of Education and Research (BMBF)
Projektname : -
Grant ID : TE 1209/1-1
Förderprogramm : -
Förderorganisation : Deutsche Forschungsgemeinschaft (DFG)

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Titel: Magnetic Resonance Materials in Physics, Biology and Medicine
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam : No longer published by Elsevier
Seiten: - Band / Heft: 32 (1) Artikelnummer: - Start- / Endseite: 105 - 144 Identifikator: ISSN: 0968-5243
CoNE: https://pure.mpg.de/cone/journals/resource/954926245532