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  Volume flows across gallbladder epithelium induced by small hydrostatic and osmotic gradients

van Os, C. H., Wiedner, G., & Wright, E. M. (1979). Volume flows across gallbladder epithelium induced by small hydrostatic and osmotic gradients. Journal of Membrane Biology, 49(1), 1-20. doi:10.1007/BF01871037.

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 Urheber:
van Os, Carel H.1, 2, Autor           
Wiedner, Günther1, 2, Autor           
Wright, Ernest M.1, 2, Autor           
Affiliations:
1Department of Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_3264819              
2Department of Physiology, University of Nijmegen, Nijmegen, The Netherlands, ou_persistent22              

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Schlagwörter: Inulin; Volume Flow; Polyethylene Glycol; Polyethylene Glycol 4000; Osmotic Gradient
 Zusammenfassung: The hydraulic conductivity of rabbit gallbladder epithelium has been studied using a continuous volumetric method based on capacitance measurements. The time resolution for measuring osmotic flows is in the range of seconds. Volume flows have been induced by osmotic gradients between 0 and 100 mosmol. In this range the flow-force relation is linear and the Pf value is 9.3×10−3 cm/sec. After correction for solute polarization effects, the Pf value amounts to 0.05 cm/sec. The observed flow is constant between 5 sec up to 20 min after a sudden increase in the osmolarity of the mucosal solution. The wet weight of the gallbladder tissue decreases by 22% and increases by 30% during osmotic flows from serosa to mucosa and from mucosa to serosa, respectively. Volume flows induced by hydrostatic pressure gradients on the mucosal surface are linearly related to the driving forces between 0 and 40 mbar. The Pf value is 0.15 cm/sec. The volume flows are constant between 2 sec and 15 min after pressure application. The flow-force relation for pressure gradients on the serosal surface is markedly nonlinear for gradients greater than 5 mbar. Below 5 mbar the Pf value is 4.5 cm/sec. From electrical measurements, e.g., resistance and streaming potentials, and from flux studies with inulin and polyethylene glycol 4000, it is concluded that hydrostatic and osmotic gradients are not comparable when they are applied to gallbladder epithelium. They induce volume flows across different pathways, e.g., osmosis predominantly across the cellular route and pressure filtration predominantly across paracellular routes.

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Sprache(n): eng - English
 Datum: 1978-12-151979-03
 Publikationsstatus: Erschienen
 Seiten: 20
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1007/BF01871037
PMID: 480337
 Art des Abschluß: -

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Titel: Journal of Membrane Biology
  Andere : J. Membr. Biol.
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: New York : Springer-Verlag New York
Seiten: - Band / Heft: 49 (1) Artikelnummer: - Start- / Endseite: 1 - 20 Identifikator: ISSN: 0022-2631
CoNE: https://pure.mpg.de/cone/journals/resource/954925415943