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  Structure of the E. coli ribosome–EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM.

Fischer, N., Neumann, P., Konevega, A. L., Bock, L. V., Ficner, R., Rodnina, M. V., et al. (2015). Structure of the E. coli ribosome–EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM. Nature, 520(7548), 567-570. doi:10.1038/nature14275.

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Fischer, N.1, Author           
Neumann, P., Author
Konevega, A. L.2, Author           
Bock, L. V.3, Author           
Ficner, R., Author
Rodnina, M. V.2, Author           
Stark, H.1, Author           
Affiliations:
1Research Group of 3D Electron Cryo-Microscopy, MPI for Biophysical Chemistry, Max Planck Society, ou_578577              
2Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
3Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              

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 Abstract: Single particle electron cryomicroscopy (cryo-EM) has recently made significant progress in high-resolution structure determination of macromolecular complexes due to improvements in electron microscopic instrumentation and computational image analysis. However, cryo-EM structures can be highly non-uniform in local resolution1, 2 and all structures available to date have been limited to resolutions above 3 Å3, 4. Here we present the cryo-EM structure of the 70S ribosome from Escherichia coli in complex with elongation factor Tu, aminoacyl-tRNA and the antibiotic kirromycin at 2.65–2.9 Å resolution using spherical aberration (Cs)-corrected cryo-EM. Overall, the cryo-EM reconstruction at 2.9 Å resolution is comparable to the best-resolved X-ray structure of the E. coli 70S ribosome5 (2.8 Å), but provides more detailed information (2.65 Å) at the functionally important ribosomal core. The cryo-EM map elucidates for the first time the structure of all 35 rRNA modifications in the bacterial ribosome, explaining their roles in fine-tuning ribosome structure and function and modulating the action of antibiotics. We also obtained atomic models for flexible parts of the ribosome such as ribosomal proteins L9 and L31. The refined cryo-EM-based model presents the currently most complete high-resolution structure of the E. coli ribosome, which demonstrates the power of cryo-EM in structure determination of large and dynamic macromolecular complexes.

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Language(s): eng - English
 Dates: 2015-02-232015-04-23
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nature14275
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 520 (7548) Sequence Number: - Start / End Page: 567 - 570 Identifier: -