hide
Free keywords:
External RBS; HeLa cells; Quantum dot; Internalization
Abstract:
We have developed an external RBS/PIXE setup at University of Gottingen. By using this setup, the analysis can be performed in atmospheric condition, which leads to realizing a quantitative analysis for volatile samples with depth information. We applied this technique for HeLa cells, internalized CdSe quantum dots (Q-dot). External RBS shows the depth difference of Q-dot depth distribution in cells, w or w/o the treatment with an endocytosis inhibitor, which agrees well with the result of confocal microscopy. The quantity of Q-dot is also evaluated by external PIXE, which coincides with the result of RBS and fluorescence measurement. In addition, the application of Si3N4 film as a medium for HeLa cells is presented.
