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  On the fundamental imaging-depth limit in two-photon microsopy

Theer, P., & Denk, W. (2006). On the fundamental imaging-depth limit in two-photon microsopy. Journal of the Optical Society of America. A, Optics, image science, and vision, 23(12), 3139-3149. doi:10.1364/JOSAA.23.003139.

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Genre: Journal Article
Alternative Title : On the fundamental imaging-depth limit in two-photon microsopy

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JOptSocAmA_23_2006_3139.pdf (Any fulltext), 800KB
 
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 Creators:
Theer, Patrick1, Author              
Denk, Winfried1, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: Fluorescence microscopy, Multiphoton processes, Optical amplifiers, Scanning microscopy, Three-dimensional microscopy, Turbid media
 Abstract: We have analyzed how the maximal imaging depth of two-photon microscopy in scattering samples depends on properties of the sample and the imaging system. We find that the imaging depth increases with increasing numerical aperture and staining inhomogeneity and with decreasing excitation-pulse duration and scattering anisotropy factor, but is ultimately limited by near-surface fluorescence with slight improvements possible using special detection strategies.

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Language(s): eng - English
 Dates: 2006-04-122006-06-262006-12-012006-12-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665344
DOI: 10.1364/JOSAA.23.003139
URI: https://www.ncbi.nlm.nih.gov/pubmed/17106469
Other: 6668
 Degree: -

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Title: Journal of the Optical Society of America. A, Optics, image science, and vision
  Other : J. Opt. Soc. Am. A-Opt. Image Sci. Vis.
Source Genre: Journal
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Publ. Info: Washington, D.C. : Optical Society of America
Pages: - Volume / Issue: 23 (12) Sequence Number: - Start / End Page: 3139 - 3149 Identifier: ISSN: 1084-7529