English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Arginine and Macrophage Activation

Comalada, M., Yeramian, A., Modolell, M., Lloberas, J., & Celeba, A. (2012). Arginine and Macrophage Activation. Methods in Molecular Biology, 844, 223-235.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Comalada, Monica, Author
Yeramian, Andree, Author
Modolell, Manuel1, Author              
Lloberas, Jorge, Author
Celeba, Antonio, Author
Affiliations:
1Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              

Content

show
hide
Free keywords: Cationinc amino acid transporters; Heterodimeric amino acid transporters; Polyamines; Cirtuline; Alternative activation; Classical activation
 Abstract: In order to perform their functions, macrophages must be activated either by Th1-type cytokines, such as interferon-gamma which is called classical activation or M1, or by Th2-type cytokines, such as IL-4, IL-10, IL-13, etc. referred as alternative activation or M2. In all of these conditions, macrophages require the uptake of exogenous arginine to meet their metabolic demands. Depending on the intracellular availability of this amino acid, the activities of these cells are differentially modulated. In this regard, macrophage activation requires this amino acid for the synthesis of proteins, production of nitric oxide via classical activation, and production of polyamines and proline through alternative activation. Therefore, the study of the arginine transport for amino acid system transporters may be a key regulatory step for physiological responses in macrophages. In this chapter, we present simple and direct methods to determine the mRNA expression and activity of arginine transporters. Moreover, we describe a direct method to measure the arginine catabolism using thin-layer chromatography.

Details

show
hide
Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 629750
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods in Molecular Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 844 Sequence Number: - Start / End Page: 223 - 235 Identifier: -