English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Obstructor-A organizes matrix assembly at the apical cell surface to promote enzymatic cuticle maturation in Drosophila.

Pesch, Y. Y., Riedel, D., & Behr, M. (2015). Obstructor-A organizes matrix assembly at the apical cell surface to promote enzymatic cuticle maturation in Drosophila. The Journal of Biological Chemistry, 290(16), 10071-10082. doi:10.1074/jbc.M114.614933.

Item is

Files

show Files
hide Files
:
2111705.pdf (Publisher version), 8MB
Name:
2111705.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Pesch, Y. Y., Author
Riedel, D.1, Author           
Behr, M., Author
Affiliations:
1Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

Content

show
hide
Free keywords: -
 Abstract: Assembly and maturation of the apical extracellular matrix (aECM) is crucial for protecting organisms, but underlying molecular mechanisms remain poorly understood. Epidermal cells secrete proteins and enzymes that assemble at the apical cell surface to provide epithelial integrity and stability during developmental growth and upon tissue damage. We analyzed molecular mechanisms of aECM assembly and identified the conserved chitin-binding protein Obstructor (Obst)-A as an essential regulator. We show in Drosophila that Obst-A is required to coordinate protein- and chitin-matrix packaging at the apical cell surface during development. Secreted by epidermal cells, the Obst-A protein is specifically enriched in the apical assembly zone where matrix components are packaged into their highly ordered architecture. In obst-A null mutant larvae, the assembly zone is strongly diminished resulting in severe disturbance of matrix-scaffold organization and impaired aECM integrity. Furthermore, enzymes that support aECM stability are mislocalized. As a biological consequence, cuticle architecture, integrity and function are disturbed in obst-A mutants finally resulting in immediate lethality upon wounding. Our studies identify a new core-organizing center, the assembly zone that controls aECM assembly at the apical cell surface. We propose a genetically conserved molecular mechanism by which Obst-A forms a matrix-scaffold to coordinate trafficking and localization of proteins and enzymes in the newly deposited aECM. This mechanism is essential for maturation and stabilization of the aECM in a growing and remodeling epithelial tissue as an outermost barrier.

Details

show
hide
Language(s): eng - English
 Dates: 2015-03-032015-04-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1074/jbc.M114.614933
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Biological Chemistry
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 290 (16) Sequence Number: - Start / End Page: 10071 - 10082 Identifier: -