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  Type 3 Fimbriae Encoded on Plasmids Are Expressed from a Unique Promoter without Affecting Host Motility, Facilitating an Exceptional Phenotype That Enhances Conjugal Plasmid Transfer

Madsen, J. S., Riber, L., Kot, W., Basfeld, A., Burmolle, M., Hansen, L. H., et al. (2016). Type 3 Fimbriae Encoded on Plasmids Are Expressed from a Unique Promoter without Affecting Host Motility, Facilitating an Exceptional Phenotype That Enhances Conjugal Plasmid Transfer. PLoS One, 11(9): e0162390. doi:10.1371/journal.pone.0162390.

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Madsen, Jonas Stenlokke1, Autor
Riber, Leise1, Autor
Kot, Witold1, Autor
Basfeld, Alrun2, Autor           
Burmolle, Mette1, Autor
Hansen, Lars Hestbjerg1, Autor
Sorensen, Soren Johannes1, Autor
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1external, ou_persistent22              
2Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              

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Schlagwörter: ESCHERICHIA-COLI K-12; CYCLIC DI-GMP; BIOFILM FORMATION; KLEBSIELLA-PNEUMONIAE; GENE-EXPRESSION; H-NS; MOLECULAR CHARACTERIZATION; ANTIMICROBIAL RESISTANCE; PSEUDOMONAS-AERUGINOSA; NUCLEOTIDE-SEQUENCEScience & Technology - Other Topics;
 Zusammenfassung: Horizontal gene transfer (HGT), the transmission of genetic material to a recipient that is not the progeny of the donor, is fundamental in bacterial evolution. HGT is often mediated by mobile genetic elements such as conjugative plasmids, which may be in conflict with the chromosomal elements of the genome because they are independent replicons that may petition their own evolutionary strategy. Here we study differences between type 3 fimbriae encoded on wild type plasmids and in chromosomes. Using known and newly characterized plasmids we show that the expression of type 3 fimbriae encoded on plasmids is systematically different, as MrkH, a c-di-GMP dependent transcriptional activator is not needed for strong expression of the fimbriae. MrkH is required for expression of type 3 fimbriae of the Klebsiella pneumoniae chromosome, where from the fimbriae operon (mrkABCDF) of plasmids is believed to have originated. We find that mrkABCDFs of plasmids are highly expressed via a unique promoter that differs from the original Klebsiella promoter resulting in fundamental behavioral consequences. Plasmid associated mrkABCDFs did not influence the swimming behavior of the host, that hereby acquired an exceptional phenotype being able to both actively swim (planktonic behavior) and express biofilm associated fimbriae (sessile behavior). We show that this exceptional phenotype enhances the conjugal transfer of the plasmid.

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Sprache(n): eng - English
 Datum: 2016-09-14
 Publikationsstatus: Online veröffentlicht
 Seiten: 19
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000383680600032
DOI: 10.1371/journal.pone.0162390
 Art des Abschluß: -

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Titel: PLoS One
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: San Francisco, CA : Public Library of Science
Seiten: - Band / Heft: 11 (9) Artikelnummer: e0162390 Start- / Endseite: - Identifikator: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850