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  Unlocking cancer glycomes from histopathological formalin-fixed and paraffin-embedded (FFPE) tissue microdissections

Hinneburg, H., Korać, P., Schirmeister, F., Gasparov, S., Seeberger, P. H., Zoldoš, V., et al. (2017). Unlocking cancer glycomes from histopathological formalin-fixed and paraffin-embedded (FFPE) tissue microdissections. Molecular and Cellular Proteomics, 16(4), 524-536. doi:10.1074/mcp.M116.062414.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-513F-4 Versions-Permalink: https://hdl.handle.net/21.11116/0000-0006-5921-1
Genre: Zeitschriftenartikel

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Hinneburg, Hannes1, Autor           
Korać, Petra, Autor
Schirmeister, Falko1, Autor           
Gasparov, Slavko, Autor
Seeberger, Peter H.2, Autor           
Zoldoš, Vlatka, Autor
Kolarich, Daniel1, Autor           
Affiliations:
1Daniel Kolarich, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863301              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              

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 Zusammenfassung: N- and O-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco-biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin-embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitative N- and O-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneous N- and O-glycomics from histopathological slides. As few as 2,000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in-depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS. N- and O-glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared to fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.

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 Datum: 2017-01-252017
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1074/mcp.M116.062414
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Titel: Molecular and Cellular Proteomics
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: Bethesda, MD : American Society for Biochemistry and Molecular Biology
Seiten: - Band / Heft: 16 (4) Artikelnummer: - Start- / Endseite: 524 - 536 Identifikator: ISSN: 1535-9476