Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Cell-Penetrating Peptides and Peptide Nucleic Acid-Coupled MRI Contrast Agents: Evaluation of Cellular Delivery and Target Binding

Mishra, R., Su, W., Pohmann, R., Pfeuffer, J., Sauer, M., Ugurbil, K., et al. (2009). Cell-Penetrating Peptides and Peptide Nucleic Acid-Coupled MRI Contrast Agents: Evaluation of Cellular Delivery and Target Binding. Bioconjugate Chemistry, 20(10), 1860-1868. doi:10.1021/bc9000454.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://pubs.acs.org/doi/abs/10.1021/bc9000454 (Verlagsversion)
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Mishra, R1, 2, Autor           
Su, W1, 2, Autor           
Pohmann, R1, 2, Autor           
Pfeuffer, J, Autor           
Sauer , MG, Autor
Ugurbil, K, Autor
Engelmann, J1, 2, Autor           
Affiliations:
1Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              
2Former Department MRZ, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528700              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Molecular imaging of cells and cellular processes can be achieved by tagging intracellular targets such as receptors, enzymes, or mRNA. Seeking to visualize the presence of
specific mRNAs by magnetic resonance (MR) imaging, we coupled peptide nucleic acids (PNA) with gadolinium-based MR contrast agents using cell-penetrating peptides for
intracellular delivery. Antisense to mRNA of DsRed2 protein was used as proof of principle. The conjugates were produced by continuous solid-phase synthesis followed by
chelation with gadolinium. Their cellular uptake was confirmed by fluorescence microscopy and spectroscopy as well as by MR imaging of labeled cells. The cell-penetrating
peptide D-Tat57amp;amp;8722;49 was selected over two other derivatives of HIV-1 Tat peptide, based on its superior intracellular delivery of the gadolinium-based contrast agents. Further
improved delivery of conjugates was achieved upon coupling
peptide nucleic acids (antisense to mRNA of DsRed2 protein and nonsense with no natural counterpart). Significant enhancement in MR contrast was obtained in cells labeled
with concentrations as low as 2.5 amp;amp;956;M of these agents. Specific binding of the targeting PNA containing conjugate to its complementary oligonucleotide sequence was proven
by in vitro cell-free assay. In contrast, a lack of specific enrichment was observed in transgenic cells containing the target due to nonspecific vesicular entrapment of contrast
agents. Preliminary biodistribution studies showed conjugate-related fluorescence in several organs, especially the liver and bladder, indicating high mobility of the agent in
spite of its high molecular weight. No conjugate related toxicity was observed. These results are encouraging, as they warrant further molecular optimization and consecutive
specificity studies in vivo of this new generation of contrast agents.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2009-09
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1021/bc9000454
BibTex Citekey: 6119
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Bioconjugate Chemistry
  Kurztitel : Bioconjugate Chem.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, D.C. : American Chemical Society
Seiten: - Band / Heft: 20 (10) Artikelnummer: - Start- / Endseite: 1860 - 1868 Identifikator: ISSN: 1043-1802
CoNE: https://pure.mpg.de/cone/journals/resource/954925589391