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  Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

Faizi, H. A., Reeves, C. J., Georgiev, V., Vlahovska, P. M., & Dimova, R. (2020). Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy. bioRxiv. doi:10.1101/2020.05.20.104240.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0006-769D-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0006-769E-4
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Faizi, Hammad A., Author
Reeves, Cody J., Author
Georgiev, Vasil1, Author           
Vlahovska, Petia M., Author
Dimova, Rumiana1, Author           
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1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

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 Abstract: A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.Competing Interest StatementThe authors have declared no competing interest.

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Language(s): eng - English
 Dates: 2020-05-222020
 Publication Status: Issued
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 Rev. Type: No review
 Identifiers: DOI: 10.1101/2020.05.20.104240
BibTex Citekey: Faizi2020.05.20.104240
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Title: bioRxiv
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Publ. Info: Cold Spring Harbor Laboratory
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