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L−745,870 PD168077 schizophrenia SKF38393 stratum radiatum
Abstract:
Dopamine plays an important role in synaptic plasticity and learning and is involved in the pathogenesis of various neurological and psychiatric disorders. Here, we reveal staining of dopaminergic fibers in stratum oriens of the mouse hippocampal CA1 region, a finding that is consistent with earlier reports. Furthermore, we examined the effect of dopamine agonists on NMDAR−dependent early long−term potentiation (LTP) (40 min) during γ−aminobutyric acid (GABA)A−mediated blockade. LTP of the AMPA component was strongly reduced in stratum oriens but barely affected in stratum radiatum. This layer−specific effect was caused by D4 receptor activation, which augmented the inactivation of synaptic NMDAR−mediated currents (NMDA EPSCs) during LTP induction through a Ca2+−dependent G−protein−independent mechanism. A similar dopaminergic modulation of both NMDA EPSCs and LTP was also observed in mice constitutively lacking NR2A but was absent in mice lacking NR2B in principal forebrain neurons. Together, these experiments strongly indicate that dopaminergic modulation of early LTP in stratum oriens occurs through NMDARs containing NR2B subunits via D4Rs. Thus, a dopamine hyperfunction in stratum oriens may result in NMDAR hypofunction that could affect both normal and pathological conditions
