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  [1,6-α-Aminosuberic acid, 3-(p-azidophenylalanine),8-arginine]vasopressin as a photoaffinity label for renal vasopressin receptors: An evaluation

Fahrenholz, F., & Crause, P. (1984). [1,6-α-Aminosuberic acid, 3-(p-azidophenylalanine),8-arginine]vasopressin as a photoaffinity label for renal vasopressin receptors: An evaluation. Biochemical and Biophysical Research Communications, 122(3), 974-982. doi:10.1016/0006-291X(84)91187-2.

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 Creators:
Fahrenholz, Falk1, Author              
Crause, Peter1, Author              
Affiliations:
1Department of Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_3264819              

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 Abstract: The photoreactive analog of vasopressin [1,6-α-aminosuberic acid, 3-(p-azidophenylalanine),8-arginine]vasopressin was labeled with tritium (specific radioactivity: 39 Ci/mmole). In the absence of UV-light, the tritium-labeled ligand retained a high binding affinity to the vasopressin receptor in plasma membranes from bovine kidney inner medulla (apparent dissociation constant KD: 1.4 × 10-8 M). Renal plasma membranes were irradiated under conditions of a high ratio of specific versus non-specific binding of the reactive ligand. Sodium dodecyl sulfate gel electrophoresis after solubilization and reduction demonstrated the preferential labeling of a polypeptide with an apparent molecular weight of Mr = 32 000. A comparison with stained gels revealed that this protein is a minor constitutent of the bovine kidney membrane. Our results suggest the 32 000-dalton polypeptide is either a component of the vasopressin receptor or that it is located in the immediate vicinity of the vasopressin binding protein.

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Language(s): eng - English
 Dates: 1984-06-222004-10-291984-08-16
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0006-291X(84)91187-2
PMID: 6089787
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 122 (3) Sequence Number: - Start / End Page: 974 - 982 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1