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  Analyzing the mesoscopic structure of pericellular coats on living cells

Böhm, H., Mundinger, T. A., Hagel, V., Boehm, C., Curtis, J. E., & Spatz, J. P. (2010). Analyzing the mesoscopic structure of pericellular coats on living cells. In M. J. Buehler, D. Kaplan, C. T. Lim, & J. Spatz (Eds.), Biological Materials and Structures in Physiologically Extreme Conditions and Disease (pp. 19-24). Warrendale, PA, USA: MRS. Retrieved from https://www.cambridge.org/core/journals/mrs-online-proceedings-library-archive/article/div-classtitleanalyzing-the-mesoscopic-structure-of-pericellular-coats-on-living-cellsdiv/4A98A2AB6F3579E1CCA54F68C21F10BC.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-3C47-4 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-7CDD-2
Genre: Conference Paper

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https://doi.org/10.1557/PROC-1274-QQ02-03 (Any fulltext)
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 Creators:
Böhm, Heike1, 2, Author           
Mundinger, Tabea A.1, 2, Author           
Hagel, Valentin, Author
Boehm, Christian1, 2, Author           
Curtis, Jennifer E.1, Author           
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: We employed passive particle-tracking microrheology to map the micromechanical structure of the hyaluronan-rich pericellular coat enveloping chondrocytes. Therefor we exploited the technique's position sensitivity to gain radial information on the coat. We observed a linear increase in viscoelasticity from the coat's rim towards the cell membrane. This gradient corresponds to hyaluronan concentration profiles observed in confocal fluorescent microscopy with small, specific hyaluronan markers. These results suggest that the structural basis of the pericellular coat is formed by grafted hyaluronan of different effective lengths stretched out by a homogenous decoration with hyaladherins such as aggrecan. The different effective lengths could be caused either by different lengths of the HA chains or by “side-on” attachments within the chain. Remarkably, the hyaluronan-rich coat increases the viscosity of the pericellular space only by about a factor of about two at 100 and at 20 Hz compared to pure media and an increasing elastic component is observed. Both the viscoelasticity as well as the hyaluronan concentration decrease linearly or slightly exponential from the cell membrane towards the PCC's rim. These observations could be obtained on living cells exploiting this unintrusive measurement techniques.

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Language(s): eng - English
 Dates: 2011-02-012011-02-012010
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 522155
URI: https://www.cambridge.org/core/journals/mrs-online-proceedings-library-archive/article/div-classtitleanalyzing-the-mesoscopic-structure-of-pericellular-coats-on-living-cellsdiv/4A98A2AB6F3579E1CCA54F68C21F10BC
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Title: MRS
Place of Event: San Francisco, CA, USA
Start-/End Date: 2010-04-05 - 2010-04-09

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Title: Biological Materials and Structures in Physiologically Extreme Conditions and Disease
Source Genre: Proceedings
 Creator(s):
Buehler, M. J.1, Editor           
Kaplan, D., Editor
Lim, C. T., Editor
Spatz, J.2, Editor           
Affiliations:
1 Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society, ou_1497656            
2 Dept. New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Max Planck Society, ou_1497649            
Publ. Info: Warrendale, PA, USA : MRS
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 19 - 24 Identifier: -

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Title: MRS Symposium Proceedings
Source Genre: Series
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Pages: - Volume / Issue: 1274 Sequence Number: 1274-QQ02-03 Start / End Page: - Identifier: -