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  Differentiation and localization of interneurons in the developing spinal cord depends on DOT1L expression

de Cristoforis, A. G., Ferrari, F., Clotman, F., & Vogel, T. (2020). Differentiation and localization of interneurons in the developing spinal cord depends on DOT1L expression. Molecular Brain, 13. doi:org/10.1186/s13041-020-00623-3.

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Gray et al. 2020.pdf (Publisher version), 20MB
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 Creators:
de Cristoforis, Angelica Gray1, Author
Ferrari, Francesco1, Author
Clotman, Frédéric2, Author
Vogel, Tanja2, Author
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              
2External Organizations, ou_persistent22              

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Free keywords: Spinal cord, Interneuron, Specification, Localization, Methyltransferase, DOT1L, Epigenetics
 Abstract: Genetic and epigenetic factors contribute to the development of the spinal cord. Failure in correct exertion of the developmental programs, including neurulation, neural tube closure and neurogenesis of the diverse spinal cord neuronal subtypes results in defects of variable severity. We here report on the histone methyltransferase Disruptor of Telomeric 1 Like (DOT1L), which mediates histone H3 lysine 79 (H3K79) methylation. Conditional inactivation of DOT1L using Wnt1-cre as driver (Dot1l-cKO) showed that DOT1L expression is essential for spinal cord neurogenesis and localization of diverse neuronal subtypes, similar to its function in the development of the cerebral cortex and cerebellum. Transcriptome analysis revealed that DOT1L deficiency favored differentiation over progenitor proliferation. Dot1l-cKO mainly decreased the numbers of dI1 interneurons expressing Lhx2. In contrast, Lhx9 expressing dI1 interneurons did not change in numbers but localized differently upon Dot1l-cKO. Similarly, loss of DOT1L affected localization but not generation of dI2, dI3, dI5, V0 and V1 interneurons. The resulting derailed interneuron patterns might be responsible for increased cell death, occurrence of which was restricted to the late developmental stage E18.5. Together our data indicate that DOT1L is essential for subtype-specific neurogenesis, migration and localization of dorsal and ventral interneurons in the developing spinal cord, in part by regulating transcriptional activation of Lhx2.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: org/10.1186/s13041-020-00623-3
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Title: Molecular Brain
Source Genre: Journal
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Publ. Info: London : BioMed Central
Pages: - Volume / Issue: 13 Sequence Number: - Start / End Page: - Identifier: ISSN: 1756-6606
CoNE: https://pure.mpg.de/cone/journals/resource/1756-6606