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  Differential messenger RNA gradients in the unicellular alga Acetabularia acetabulum. Role of the cytoskeleton

Vogel, H., Grieninger, G. E., & Zetsche, K. (2002). Differential messenger RNA gradients in the unicellular alga Acetabularia acetabulum. Role of the cytoskeleton. Plant Physiology, 129(3), 1407-1416. doi:10.1104/pp.010983.

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Vogel, H.1, Author           
Grieninger, G. E., Author
Zetsche, K., Author
Affiliations:
1Group of Genetics and Evolution, MPI for Chemical Ecology, Max Planck Society, ou_421896              

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Free keywords: CALMODULIN-BINDING PROTEINS LOCALIZATION MICROFILAMENTS ISOFORMS PLANTS BODY
 Abstract: The unicellular green alga Acetabularia acetabulum has proven itself to be a superior model for studies of morphogenesis because of its large size and distinctive polar morphology. The giant cell forms an elongated tube (a stalk of up to 60 mm in length), which at its apical pole makes whorls of hairs, followed by one whorl of gametophores in the shape of a cap. At its basal pole, the cell extends into a rhizoid wherein the single nucleus is positioned. In this study, we have determined the level of specific messenger RNAs in the apical, middle, and basal regions using reverse transcriptase-PCR methodology. Four mRNA classes were distinguished: those that were
uniformly distributed (small subunit of Rubisco, actin-1, ADPglucose centrin, and alpha- and beta-tubulin), those that expressed apical/basal (calmodulin-4) or basal/apical
gradients (calmodulin-2 and a Ran-G protein), and those with development-specific patterns of distribution (mitogen-activated protein kinase, actin-2, and
UDP-glucose-epimerase). Restoration of the apical/basal calmodulin-4 mRNA gradient after amputation of the apical region of the cell requires the nucleus and was
abolished by cytochalasin D. Accumulation of actin-1 mRNA in the vicinity of the wound set by the amputation needs, likewise, the presence of the nucleus and was also
inhibited by cytochalasin. This suggests that actin microfilaments of the cytoskeleton are involved in directed transport and/or anchoring of these mRNAs.

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 Dates: 2002
 Publication Status: Issued
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 Identifiers: Other: MIO135
DOI: 10.1104/pp.010983
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 129 (3) Sequence Number: - Start / End Page: 1407 - 1416 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438