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  Structural evidence that myosin heads may interact with two sites on F-actin

Amos, L. A., Huxley, H. E., Holmes, K. C., & Goody, R. S. (1982). Structural evidence that myosin heads may interact with two sites on F-actin. Nature, 299(5882), 467-469. doi:10.1038/299467a0.

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Amos, L. A., Author
Huxley, Hugh E., Author
Holmes, Kenneth C.1, 2, 3, Author           
Goody, Roger S.2, Author           
Affiliations:
1Protein Cristallography XDS, Max Planck Institute for Medical Research, Max Planck Society, ou_1497735              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
3Muscle Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497731              

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 Abstract: Analysis of the rigor complex of muscle thin filaments decorated with myosin subfragment-1 (S-1) by three-dimensional image reconstruction from electron micrographs1 shows S-1 to be a comma-shaped molecule, the broad head of which interacts with F-actin near the groove between the two strands of actin monomers. Increased resolution near the filament axis has allowed us to distinguish separate components more reliably than in an earlier analysis2. Using X-ray diffraction data from striated muscle decorated with S-1 molecules3 as a reference, we have now refined the electron microscope data to show the interaction in more detail. We show here that individual S-1 molecules apparently interact with both strands of a filament, straddling the regulatory protein tropomyosin in the long-pitch groove. This double-sited interaction agrees with chemical cross-linking data4 and suggests some interesting possibilities for cross-bridge cycling in muscle.

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Language(s): eng - English
 Dates: 1982-05-121982-07-161982-09-30
 Publication Status: Issued
 Pages: 3
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 Rev. Type: Peer
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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 299 (5882) Sequence Number: - Start / End Page: 467 - 469 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238