Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Simple and efficient system for photoconverting light-sensitive proteins in serial crystallography experiments

Schirò, G., Woodhouse, J., Weik, M., Schlichting, I., & Shoeman, R. L. (2017). Simple and efficient system for photoconverting light-sensitive proteins in serial crystallography experiments. Journal of Applied Crystallography, 50, 932-939. doi:10.1107/S1600576717006264.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-6F6F-A Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-6F70-4
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
JApplCryst_50_2017_932.pdf (beliebiger Volltext), 617KB
 
Datei-Permalink:
-
Name:
JApplCryst_50_2017_932.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
JApplCryst_50_2017_932_Suppl.pdf (Ergänzendes Material), 351KB
 
Datei-Permalink:
-
Name:
JApplCryst_50_2017_932_Suppl.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, MHMF; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
http://journals.iucr.org/j/issues/2017/03/00/te5018/te5018.pdf (beliebiger Volltext)
Beschreibung:
-
OA-Status:
externe Referenz:
https://doi.org/10.1107/S1600576717006264 (beliebiger Volltext)
Beschreibung:
-
OA-Status:
externe Referenz:
https://doi.org/10.1107/S1600576717006264/te5018sup1.pdf (Ergänzendes Material)
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Schirò, Giorgio, Autor
Woodhouse, Joyce, Autor
Weik, Martin, Autor
Schlichting, Ilme1, Autor           
Shoeman, Robert L.1, Autor           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Inhalt

einblenden:
ausblenden:
Schlagwörter: serial femtosecond crystallography; light-sensitive proteins; pre-illumination; time resolution; instrumentation
 Zusammenfassung: Proteins that change their structure in response to light absorption regulate many functional processes in living cells. Moreover, biotechnological approaches like optogenetics and super-resolution fluorescence microscopy recently triggered the generation of new genetically modified photosensitive proteins. Light-induced structural changes in photosensitive proteins can be studied by time-resolved serial femtosecond crystallography (SFX), an X-ray diffraction technique that allows the determination of macromolecular structures at X-ray free-electron lasers from a large number of nano- to micro-sized crystals. This article describes a simple and efficient system for converting photosensitive proteins into light-induced semi-stationary states by inline laser illumination prior to sample injection with a gas-focused liquid jet and subsequent optical pump–X-ray probe exposure. The simple setup of this device makes it suitable for integration into other liquid injectors (like electro-spinning and electro-kinetic injectors) and potentially also in high-viscosity extruders, provided that embedding microcrystals in viscous media does not alter protein photophysical properties. The functioning of the device is demonstrated with an example of a photoswitchable fluorescent protein pre-illuminated (photoactivated) for time-resolved SFX experiments. The device can be easily adapted for the conversion in time-resolved SFX experiments of other microcrystalline proteins, such as photosystems, phytochromes and rhodopsins.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2016-12-132017-04-252017
 Publikationsstatus: Erschienen
 Seiten: 8
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1107/S1600576717006264
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of Applied Crystallography
  Kurztitel : J. Appl. Cryst.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Oxford, England : Blackwell Publishing on behalf of the International Union of Crystallography
Seiten: - Band / Heft: 50 Artikelnummer: - Start- / Endseite: 932 - 939 Identifikator: ISSN: 0021-8898
CoNE: https://pure.mpg.de/cone/journals/resource/954925410812