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  Fluorogenic probes for live-cell imaging of the cytoskeleton.

Lukinavicius, G., Reymond, L., D'Este, E., Masharina, A., Göttfert, F., Ta, H., et al. (2014). Fluorogenic probes for live-cell imaging of the cytoskeleton. Nature Methods, 11(7), 731-733. doi:10.1038/nmeth.2972.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-A5DC-D Version Permalink: https://hdl.handle.net/21.11116/0000-0001-A5E2-5
Genre: Journal Article

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 Creators:
Lukinavicius, G.1, Author           
Reymond, L., Author
D'Este, E.2, Author           
Masharina, A., Author
Göttfert, F.2, Author           
Ta, H.2, Author           
Guether, A., Author
Fournier, M., Author
Rizzo, S., Author
Waldmann, H., Author
Blaukopf, C., Author
Sommer, C., Author
Gerlich, D. W., Author
Arndt, H. D., Author
Hell, S. W.2, Author           
Johnsson, K., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
2Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

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Language(s): eng - English
 Dates: 2014-05-252014-07
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nmeth.2972
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Title: Nature Methods
Source Genre: Journal
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Pages: - Volume / Issue: 11 (7) Sequence Number: - Start / End Page: 731 - 733 Identifier: -