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  Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies

Keidel, A., Conti, E., & Falk, S. (2020). Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies. In Methods in Enzymology, 2062: Eukaryotic RNA Exosome: Methods and Protocols (pp. 491-513). HUMANA PRESS INC.

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 Creators:
Keidel, Achim1, Author              
Conti, Elena1, Author              
Falk, Sebastian1, Author
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Free keywords: CRYSTAL-STRUCTURE; RNA DEGRADATION; EXOSOME; PROTEIN; MTR4; REVEALS; BINDING; DOMAINExosome; Mtr4; Trf4; Air2; TRAMP; Ski2; Ski3; Ski8; Ski complex;
 Abstract: The RNA exosome is a macromolecular machine that degrades a large variety of RNAs from their 3'-end. It comprises the major 3'-to-5' exonuclease in the cell, completely degrades erroneous and overly abundant RNAs, and is also involved in the precise processing of RNAs. To degrade transcripts both specifically and efficiently the exosome functions together with compartment-specific cofactors. In the yeast S. cerevisiae, the exosome associates with the Ski complex in the cytoplasm and with Mtr4 alone or with Mtr4 as part of the TRAMP complex in the nucleus. Here we describe how to produce, purify, and assemble the Ski and TRAMP complexes from S. cerevisiae.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: 23
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Methods in Enzymology, 2062: Eukaryotic RNA Exosome: Methods and Protocols
  Alternative Title : Eukaryotic RNA Exosome: Methods and Protocols
Source Genre: Book
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Affiliations:
Publ. Info: HUMANA PRESS INC
Pages: - Volume / Issue: 2062 Sequence Number: - Start / End Page: 491 - 513 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-9822-7; 978-1-4939-9821-0

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Title: Methods in Molecular Biology
  Alternative Title : METHODS MOL BIOL
  Alternative Title : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 2062 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745