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  Activity-dependent spatially localized miRNA maturation in neuronal dendrites

Sambandan, S., Akbalik, G., Kochen, L., Rinne, J., Kahlstatt, J., Glock, C., et al. (2017). Activity-dependent spatially localized miRNA maturation in neuronal dendrites. Science, 355(6325), 634-637. doi:10.1126/science.aaf8995.

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Genre: Journal Article

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Sambandan, S., Author
Akbalik, G., Author
Kochen, L., Author
Rinne, J., Author
Kahlstatt, J., Author
Glock, C., Author
Tushev, G., Author
Alvarez-Castelao, B., Author
Heckel, A., Author
Schuman, Erin M.1, Author              
Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              

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Free keywords: Animals Cells, Cultured Dendrites/*metabolism Fluorescent Dyes/chemistry *Gene Expression Regulation Hippocampus/cytology Male MicroRNAs/*metabolism Neurons/*metabolism Protein Biosynthesis/*genetics RNA Cleavage RNA, Messenger/metabolism Rats Rats, Sprague-Dawley Ribonuclease III/genetics/metabolism Synapses/metabolism
 Abstract: MicroRNAs (miRNAs) regulate gene expression by binding to target messenger RNAs (mRNAs) and preventing their translation. In general, the number of potential mRNA targets in a cell is much greater than the miRNA copy number, complicating high-fidelity miRNA-target interactions. We developed an inducible fluorescent probe to explore whether the maturation of a miRNA could be regulated in space and time in neurons. A precursor miRNA (pre-miRNA) probe exhibited an activity-dependent increase in fluorescence, suggesting the stimulation of miRNA maturation. Single-synapse stimulation resulted in a local maturation of miRNA that was associated with a spatially restricted reduction in the protein synthesis of a target mRNA. Thus, the spatially and temporally regulated maturation of pre-miRNAs can be used to increase the precision and robustness of miRNA-mediated translational repression.

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 Dates: 2017-02-12
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: 28183980
DOI: 10.1126/science.aaf8995
ISSN: 1095-9203 (Electronic)0036-8075 (Linking)
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 355 (6325) Sequence Number: - Start / End Page: 634 - 637 Identifier: -