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  Insights into the assembly and architecture of a Staufen-mediated mRNA decay (SMD)-competent mRNP.

Gowravaram, M., Schwarz, J., Khilji, S. K., Urlaub, H., & Chakrabarti, S. (2019). Insights into the assembly and architecture of a Staufen-mediated mRNA decay (SMD)-competent mRNP. Nature Communications, 10: 5054. doi:10.1038/s41467-019-13080-x.

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Gowravaram, Manjeera, Author
Schwarz, Juliane, Author
Khilji, Sana K.1, Author           
Urlaub, Henning, Author
Chakrabarti, Sutapa, Author
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1Oren Moscovitz, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3176803              

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 Abstract: The mammalian Staufen proteins (Stau1 and Stau2) mediate degradation of mRNA containing complex secondary structures in their 3'-untranslated region (UTR) through a pathway known as Staufen-mediated mRNA decay (SMD). This pathway also involves the RNA helicase UPF1, which is best known for its role in the nonsense-mediated mRNA decay (NMD) pathway.
Here we present a biochemical reconstitution of the recruitment and activation of UPF1 in context of the SMD pathway. We demonstrate the involvement of UPF2, a core NMD factor and a known activator of UPF1, in SMD. UPF2 acts as an adaptor between Stau1 and UPF1, stimulates the catalytic activity of UPF1 and plays a central role in the formation of an SMD-competent mRNP. Our study elucidates the molecular mechanisms of SMD and points towards extensive cross-talk between UPF1-mediated mRNA decay pathways in cells.

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Language(s): eng - English
 Dates: 2019-11-072019
 Publication Status: Issued
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 31699982
DOI: 10.1038/s41467-019-13080-x
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 10 Sequence Number: 5054 Start / End Page: - Identifier: ISSN: 2041-1723