Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Tissue-specific dissociation of diurnal transcriptome rhythms during sleep restriction in mice.

Husse, J., Kiehn, J. T., Barclay, J. L., Naujokat, N., Meyer-Kovac, J., Lehnert, H., et al. (2017). Tissue-specific dissociation of diurnal transcriptome rhythms during sleep restriction in mice. Sleep, 40(6): zsx068. doi:10.1093/sleep/zsx068.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
2470048.pdf (Verlagsversion), 5MB
 
Datei-Permalink:
-
Name:
2470048.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (UNKNOWN id 303; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
2470048_Suppl_1.pdf (Ergänzendes Material), 808KB
Name:
2470048_Suppl_1.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
2470048_Suppl_2.pdf (Ergänzendes Material), 437KB
Name:
2470048_Suppl_2.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
2470048_Suppl_3.pdf (Ergänzendes Material), 331KB
Name:
2470048_Suppl_3.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
2470048_Suppl_4.docx (Ergänzendes Material), 15KB
Name:
2470048_Suppl_4.docx
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Husse, J., Autor
Kiehn, J. T., Autor
Barclay, J. L., Autor
Naujokat, N., Autor
Meyer-Kovac, J., Autor
Lehnert, H., Autor
Oster, H.1, Autor           
Affiliations:
1Research Group of Circadian Rhythms, MPI for biophysical chemistry, Max Planck Society, ou_578594              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Sleep restriction; transcriptome; circadian clock; liver; mediobasal hypothalamus; white adipose tissue
 Zusammenfassung: Study objectives: Shortened or mistimed sleep affects metabolic homeostasis, which may in part be mediated by dysregulation of endogenous circadian clocks. In this study, we assessed the contribution of sleep disruption to metabolic dysregulation by analysing diurnal transcriptome regulation in metabolic tissues of mice subjected to a sleep restriction (SR) paradigm. Methods: Male mice were subjected to 2 x 5 days of SR with enforced waking during the first 6 hours of the light phase. SR and control mice were sacrificed at different time points of the day and RNA preparations from the mediobasal hypothalamus (MBH), liver, and epididymal white adipose tissue (eWAT) were subjected to whole-genome microarray hybridization. Transcriptional rhythms were associated with changes in behavioral and physiological parameters such as sleep, body temperature, and food intake. Rhythm detection was performed with CircWave and transcription profiles were compared by 2-way analysis of variance and t-tests with Benjamini-Hochberg corrections. Results: Clock gene rhythms were blunted in all tissues, while transcriptome regulation was associated with either clock gene expression, sleep patterns, or food intake in a tissue-specific manner. Clock gene expression was associated with apoptosis pathways in the MBH and with tumor necrosis factor alpha signalling in liver. Food intake-associated genes included cilium movement genes in the MBH and lipid metabolism-associated transcripts in liver. Conclusions: In mice, repeated SR profoundly alters behavioral and molecular diurnal rhythms, disrupting essential signalling pathways in MBH, liver, and eWAT, which may underlie the metabolic and cognitive disturbances observed in sleep-restricted humans such as shift workers.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2017-04-242017-06-01
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1093/sleep/zsx068
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Sleep
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: 13 Band / Heft: 40 (6) Artikelnummer: zsx068 Start- / Endseite: - Identifikator: -