Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Selective cross-linking of interacting proteins using self-labeling tags.

Gautier, A., Nakata, E., Lukinavicius, G., Tan, K. T., & Johnsson, K. (2009). Selective cross-linking of interacting proteins using self-labeling tags. Journal of the American Chemical Society, 131(49), 17954-17962. doi:10.1021/ja907818q.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
2629774.pdf (Verlagsversion), 3MB
 
Datei-Permalink:
-
Name:
2629774.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (UNKNOWN id 303; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
2629774_Suppl.pdf (Ergänzendes Material), 3MB
Name:
2629774_Suppl.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Gautier, A., Autor
Nakata, E., Autor
Lukinavicius, G.1, Autor           
Tan, K. T., Autor
Johnsson, K., Autor
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: We have designed molecules that permit the selective cross-linking (S-CROSS) of interacting proteins in cell lysates and the sensitive detection of the trapped complexes through in-gel fluorescence scanning. S-CROSS requires the expression of the putative interacting proteins as fusion to CLIP-tag or SNAP-tag, two protein tags that can be specifically labeled with synthetic probes. Bifunctional molecules that contain the substrates of the two tags connected via a fluorophore are used to selectively cross-link interacting proteins in cell lysate. The amount of trapped complex can be then quantified after SDS gel electrophoresis by in-gel fluorescence scanning. On the basis of a detailed kinetic analysis of the cross-linking reaction, we showed that the cross-linking efficiency can be used as an indicator of interaction between two proteins, allowing thereby the unambiguous identification of interacting protein pairs. We validated our approach by confirming a number of interactions through selective cross-linking and showed that it permits the quantitative and simultaneous analysis of multiple homotypic and heterotypic protein complexes and the differentiation between strong and weak protein−protein interactions.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2009-11-162009
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1021/ja907818q
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of the American Chemical Society
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 131 (49) Artikelnummer: - Start- / Endseite: 17954 - 17962 Identifikator: -