ausblenden:
Schlagwörter:
Model membranes - Artificial lipid membranes - Liposomes - Small unilamellar vesicles - Giant unilamellar vesicles - Supported lipid bilayers - Nanodiscs - Plasma membrane sheets - Receptor–ligand interaction
Zusammenfassung:
Model membrane systems can be classified according to their shape and origin. Depending on the shape, model membranes are compatible with a wide variety of methods, ranging from biochemical and biophysical solution assays to microscopy. So far, many applications have been focused on studying the membranes themselves, rather than membrane-associated processes like protein–protein or protein–ligand interactions. However, many model membranes with embedded proteins should be amenable to these studies, too, i.e., be suitable samples for many of the techniques discussed in this book. In this chapter, we discuss how some practical limitations that often restrict the use of model membranes can be overcome. After introducing various membrane structures, an overview is given over alternative ways of their production. Finally, several established protocols, which are suitable for beginners in the field, are described in detail.
