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  Leaf wax lipid extraction for archaeological applications

Patalano, R., Zech, J., & Roberts, P. (2020). Leaf wax lipid extraction for archaeological applications. Current Protocols in Plant Biology, 5(3). doi:10.1002/cppb.20114.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0006-E07B-3 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0006-E098-1
資料種別: 学術論文

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shh2688.pdf (出版社版), 2MB
 
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作成者

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 作成者:
Patalano, Robert1, 著者           
Zech, Jana1, 著者           
Roberts, Patrick1, 著者           
所属:
1Archaeology, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074312              

内容説明

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キーワード: archaeology, human evolution, isotope analysis, lipid biomarkers, paleoecology
 要旨: Plant wax lipid molecules, chiefly normal (n-) alkanes and n-alkanoic acids, are frequently used as proxies for understanding paleoenvironmental and paleoclimatic change. These are regularly analyzed from marine and lake sediments and even more frequently in archaeological contexts, enabling the reconstruction of past environments in direct association with records of past human behavior. Carbon and hydrogen isotope measurements of these compounds are used to trace plant type and water-use efficiency, relative paleotemperature, precipitation, evapotranspiration of leaf and soil moisture, and other physiological and ecological parameters. Plant wax lipids have great potential for answering questions related to human-environment interactions, being for the most part chemically inert and easily recoverable in terrestrial sediments, including those dating back millions of years. The growing use of this technique, and comparison of such data with other paleoenvironmental proxies such as pollen and phytolith analysis and soil carbonate and tooth enamel isotope records, make it essential to establish consistent, best-practice protocols for extracting n-alkanes and n-alkanoic acids from archaeological sediments to provide comparable information for interpreting past climatic, ecosystem, and hydrological changes and their interaction with human societies.

資料詳細

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言語: eng - English
 日付: 2020-08-132020-09
 出版の状態: 出版
 ページ: 38
 出版情報: -
 目次: Introduction

Basic Protocol 1: Total lipid extraction
- Materials
- Setup of Büchi SpeedExtractor E-916 Pressurized Speed Extractor (PSE)
- Creation of a new method
- Sample preparation
- Extraction process
- Post-extraction procedures

Support Protocol 1: Weighing the total lipid extract
- Materials

Support Protocol 2: Cleaning the PSE extraction cells
- Materials

Alternate Protocol 1: Soxhlet total lipid extraction
- Materials
- Precleaning of Soxhlet apparatus and consumables
- Sample preparation
- Soxhlet extraction

Alternate Protocol 2: Ultrasonic total lipid extraction
- Materials
- Sample preparation
- Ultrasonic extraction

Basic Protocol 2: Separation of lipids by aminopropyl column chromatography
- Materials

Basic Protocol 3: Separation of lipids by silver‐nitrate‐infused silica gel column chromatography
- Materials
- Column preparation
- Column chromatography

Support Protocol 3: Preparation of silica gel infused with 10% silver nitrate
- Materials

Basic Protocol 4: Methylation of n‐alkanoic acids
- Materials
- Methylation
- Isolation of FAMEs

Basic Protocol 5: Gas chromatography mass spectrometry (GC‐MS)
- Materials
- Preparing samples for GC analysis
- Setting recommended parameters for GC-MS operation
- Operating the GC-MS for sample analysis
- Identifying compounds

Basic Protocol 6: Gas chromatography isotope ratio mass spectrometry (GC‐IRMS)
- Materials
- Tuning the IRMS
- Setting recommended parameters for GC-IRMS operation
- Operating the GC-IRMS apparatus for sample analysis

Commentary
- Background information
- Critical Parameters
- Troubleshooting
- Statistical Analysis
- Understanding Results
- Time Considerations
 査読: 査読あり
 識別子(DOI, ISBNなど): DOI: 10.1002/cppb.20114
 学位: -

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Project information

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Project name : PANTROPOCENE
Grant ID : 850709
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

出版物 1

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出版物名: Current Protocols in Plant Biology
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: John Wiley & Sons, Ltd
ページ: e20114 巻号: 5 (3) 通巻号: - 開始・終了ページ: - 識別子(ISBN, ISSN, DOIなど): ISBN: 2379-8068

出版物 2

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出版物名: Current Protocols in Plant Biology
  省略形 : Curr Protoc Plant Biol
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: Chichester : Wiley
ページ: - 巻号: 5 (3) 通巻号: e20114 開始・終了ページ: - 識別子(ISBN, ISSN, DOIなど): ISSN: 2379-8068
CoNE: https://pure.mpg.de/cone/journals/resource/2379-8068