Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  An In Vivo Metabolic Approach for Deciphering the Product Specificity of Glycerate Kinase Proves that Both E. coli's Glycerate Kinases Generate 2-Phosphoglycerate

Zelcbuch, L., Razo-Mejia, M., Herz, E., Yahav, S., Antonovsky, N., Kroytoro, H., et al. (2015). An In Vivo Metabolic Approach for Deciphering the Product Specificity of Glycerate Kinase Proves that Both E. coli's Glycerate Kinases Generate 2-Phosphoglycerate. PLoS One, 10(3): e0122957. doi:10.1371/journal.pone.0122957.

Item is

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Zelcbuch, Lior1, Autor
Razo-Mejia, Manuel1, Autor
Herz, Elad1, Autor
Yahav, Sagit1, Autor
Antonovsky, Niv1, Autor
Kroytoro, Hagar1, Autor
Milo, Ron1, Autor
Bar-Even, A.2, Autor           
Affiliations:
1external, ou_persistent22              
2Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2035297              

Inhalt

einblenden:
ausblenden:
Schlagwörter: ESCHERICHIA-COLI; RECONSTRUCTION; 3-KINASE; PATHWAY; ENZYMEScience & Technology - Other Topics;
 Zusammenfassung: Apart from addressing humanity's growing demand for fuels, pharmaceuticals, plastics and other value added chemicals, metabolic engineering of microbes can serve as a powerful tool to address questions concerning the characteristics of cellular metabolism. Along these lines, we developed an in vivo metabolic strategy that conclusively identifies the product specificity of glycerate kinase. By deleting E. coli's phosphoglycerate mutases, we divide its central metabolism into an 'upper' and 'lower' metabolism, each requiring its own carbon source for the bacterium to grow. Glycerate can serve to replace the upper or lower carbon source depending on the product of glycerate kinase. Using this strategy we show that while glycerate kinase from Arabidopsis thaliana produces 3-phosphoglycerate, both E. coli's enzymes generate 2-phosphoglycerate. This strategy represents a general approach to decipher enzyme specificity under physiological conditions.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2015
 Publikationsstatus: Erschienen
 Seiten: 7
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000352134700211
DOI: 10.1371/journal.pone.0122957
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: PLoS One
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: San Francisco, CA : Public Library of Science
Seiten: - Band / Heft: 10 (3) Artikelnummer: e0122957 Start- / Endseite: - Identifikator: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850