ausblenden:
Schlagwörter:
STAM2; Gene trap; Mouse; Embryo; Expression; X-gal staining; Development
Zusammenfassung:
STAM2 is a tyrosine-phosphorylated protein suggested to be involved in cargo selection during endocytic
pathway, regulation of exocytosis and intracellular signaling. Gene trap method was used to create via
insertional mutagenesis a mutant mouse line with integration of promoterless bgeo (lacZ–neomycin phosphotransferase
fusion) gene in the second intron of Stam2 gene, enabling analysis of its in vivo expression
and function. The inserted b-galactosidase (lacZ) reporter gene was used to reveal Stam2 expression during
development. Stam2 in situ RNA hybridization and immunostaining confirmed the observed b-galactosidase
activity reflecting high Stam2 expression. The homozygous mutant mice showed no overt
phenotypic alterations. Stam2 expression was detected after E9.5 in the gut, notochord, neural tube
and heart. In the nervous system it was located in the floor, roof and basal plates of the developing neural
tube, and in the developing cortex, hippocampus and olfactory bulbs. Toward the end of gestation, Stam2
expression appeared in the testis and ovary, lungs, nasal cavity epithelium, kidneys, urogenital sinus,
intestine, pancreas, pituitary and adrenal glands, muscles, brown adipose tissue, skin and epithelium
of the tongue and oral cavity.
