Radiation damage in protein serial femtosecond crystallography using an x-ray 
free-electron laser

Lomb, Lukas; Barends, Thomas; Kassemeyer, Stephan; Aquila, Andrew; Epp, Sascha W.; Erk, Benjamin; Foucar, Lutz; Hartmann, Robert; Rudek, Benedikt; Rolles, Daniel; Rudenko, Artem; Shoeman, Robert L.; Andreasson, Jakob; Bajt, Saša; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J.; Bostedt, Christoph; Bozek, John D.; Caleman, Carl; Coffee, Ryan; Coppola, Nicola; DePonte, Daniel P.; Doak, R. Bruce; Ekeberg, Tomas; Fleckenstein, Holger; Fromme, Petra; Gebhardt, Maike; Graafsma, Heinz; Gumprecht, Lars; Hampton, Christina Y.; Hartmann, Andreas; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton1, James M.; Hunter, Mark S.; Kabsch, Wolfgang; Kimmel, Nils; Kirian, Richard A.; Liang, Mengning; Maia, Filipe R. N. C.; Meinhart, Anton; Marchesini, Stefano; Martin, Andrew V.; Nass, Karol; Reich, Christian; Schulz, Joachim; Seibert, M. Marvin; Sierra, Raymond G.; Soltau, Heike; Spence, John C. H.; Steinbrener, Jan; Stellato, Francesco; Stern, Stephan; Timneanu, Nicusor; Wang, Xiaoyu; Weidenspointner, Georg; Weierstall, Uwe; White, Thomas A.; Wunderer, Cornelia B.; Chapman, Henry N.; Ullrich, Joachim; Strüder, Lothar; Schlichting, Ilme

doi:10.1103/PhysRevB.84.214111

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Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lomb, L., Barends, T., Kassemeyer, S., Aquila, A., Epp, S. W., Erk, B., et al. (2011). Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. Physical Review B, 84(21): e214111, pp. 1-6. doi:10.1103/PhysRevB.84.214111.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-1D94-B Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-1D95-9

Genre: Journal Article

Alternative Title : Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

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Lomb, Lukas¹, Author
Barends, Thomas¹, Author
Kassemeyer, Stephan¹, Author
Aquila, Andrew, Author
Epp, Sascha W., Author
Erk, Benjamin¹, Author
Foucar, Lutz¹, Author
Hartmann, Robert, Author
Rudek, Benedikt¹, Author
Rolles, Daniel¹, Author
Rudenko, Artem, Author
Shoeman, Robert L.¹, Author
Andreasson, Jakob, Author
Bajt, Saša, Author
Barthelmess, Miriam, Author
Barty, Anton, Author
Bogan, Michael J., Author
Bostedt, Christoph, Author
Bozek, John D., Author
Caleman, Carl, Author
Coffee, Ryan, AuthorCoppola, Nicola, AuthorDePonte, Daniel P., AuthorDoak, R. Bruce¹, Author         Ekeberg, Tomas, AuthorFleckenstein, Holger, AuthorFromme, Petra, AuthorGebhardt, Maike¹, Author         Graafsma, Heinz, AuthorGumprecht, Lars, AuthorHampton, Christina Y., AuthorHartmann, Andreas, AuthorHauser, Günter, AuthorHirsemann, Helmut, AuthorHoll, Peter, AuthorHolton1, James M., AuthorHunter, Mark S., AuthorKabsch, Wolfgang¹, Author         Kimmel, Nils, AuthorKirian, Richard A., AuthorLiang, Mengning, AuthorMaia, Filipe R. N. C., AuthorMeinhart, Anton¹, Author         Marchesini, Stefano, AuthorMartin, Andrew V., AuthorNass, Karol¹, Author         Reich, Christian, AuthorSchulz, Joachim, AuthorSeibert, M. Marvin, AuthorSierra, Raymond G., AuthorSoltau, Heike, AuthorSpence, John C. H., AuthorSteinbrener, Jan¹, Author         Stellato, Francesco, AuthorStern, Stephan, AuthorTimneanu, Nicusor, AuthorWang, Xiaoyu, AuthorWeidenspointner, Georg, AuthorWeierstall, Uwe, AuthorWhite, Thomas A., AuthorWunderer, Cornelia B., AuthorChapman, Henry N., AuthorUllrich, Joachim, AuthorStrüder, Lothar, AuthorSchlichting, Ilme¹, Author          more..

Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700

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Abstract: X−ray free−electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects

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Language(s): eng - English

Dates: Submitted: 2011-07-23Date issued: 2011-12-20

Publication Status: Issued

Pages: 6

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Rev. Type: Peer

Identifiers: eDoc: 664467
DOI: 10.1103/PhysRevB.84.214111
Other: 7734

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Title: Physical Review B

Other : Phys. Rev. B

Source Genre: Journal

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Publ. Info: Woodbury, NY : American Physical Society

Pages: - Volume / Issue: 84 (21) Sequence Number: e214111 Start / End Page: 1 - 6 Identifier: ISSN: 1098-0121
CoNE: https://pure.mpg.de/cone/journals/resource/954925225008