English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening

Schilbach, S., Aibara, S., Dienemann, C., Grabbe, F., & Cramer, P. (2021). Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening. Cell, 184(15), 4064-4072.e28. doi:10.1016/j.cell.2021.05.012.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
3327757.pdf (Publisher version), 20MB
 
File Permalink:
-
Name:
3327757.pdf
Description:
-
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Schilbach, S.1, Author              
Aibara, S.1, Author              
Dienemann, C.1, Author              
Grabbe, F.1, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: pre-initiation complex; transcription initiation; transcription factors; RNA polymerase II; promoter DNA opening; promoter DNA melting; transcription bubble; in vitro transcription; cryo-electron microscopy; structure
 Abstract: Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate state that contains half a turn of open DNA located 30–35 base pairs downstream of the TATA box. The initially opened DNA region is flanked and stabilized by the polymerase “clamp head loop” and the TFIIF “charged region” that both contribute to promoter-initiated transcription. TFIIE facilitates initiation by buttressing the clamp head loop and by regulating the TFIIH translocase. The initial DNA bubble is then extended in the upstream direction, leading to the open promoter complex and enabling start-site scanning and RNA synthesis. This unique mechanism of DNA opening may permit more intricate regulation than in the Pol I and Pol III systems.

Details

show
hide
Language(s): eng - English
 Dates: 2021-06-152021-07-22
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.cell.2021.05.012
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 184 (15) Sequence Number: - Start / End Page: 4064 - 4072.e28 Identifier: -