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  The metagenome of the marine anammox bacterium 'Candidatus Scalindua profunda' illustrates the versatility of this globally important nitrogen cycle bacterium

van de Vossenberg, J., Woebken, D., Maalcke, W. J., Wessels, H. J. C. T., Dutilh, B. E., Kartal, B., et al. (2013). The metagenome of the marine anammox bacterium 'Candidatus Scalindua profunda' illustrates the versatility of this globally important nitrogen cycle bacterium. Environmental Microbiology, 15(5 Sp. Iss. SI), 1275-1289.

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van de Vossenberg, J., Author
Woebken, D.1, Author           
Maalcke, W. J., Author
Wessels, H. J. C. T., Author
Dutilh, B. E., Author
Kartal, B.2, Author           
Janssen-Megens, E. M., Author
Roeselers, G., Author
Yan, J., Author
Speth, D., Author
Gloerich, J., Author
Geerts, W., Author
van der Biezen, E., Author
Pluk, W., Author
Francoijs, K. J., Author
Russ, L., Author
Lam, P.3, Author           
Malfatti, S. A., Author
Tringe, S. G., Author
Haaijer, S. C. M., Author
Op den Camp, H. J. M., AuthorStunnenberg, H. G., AuthorAmann, R.1, Author           Kuypers, M. M. M.3, Author           Jetten, M. S. M., Author more..
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              
2Research Group for Microbial Physiology, Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481694              
3Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              

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 Abstract: Anaerobic ammonium-oxidizing (anammox) bacteria are responsible for a significant portion of the loss of fixed nitrogen from the oceans, making them important players in the global nitrogen cycle. To date, marine anammox bacteria found in marine water columns and sediments worldwide belong almost exclusively to the Candidatus Scalindua' species, but the molecular basis of their metabolism and competitive fitness is presently unknown. We applied community sequencing of a marine anammox enrichment culture dominated by Candidatus Scalindua profunda' to construct a genome assembly, which was subsequently used to analyse the most abundant gene transcripts and proteins. In the S.profunda assembly, 4756 genes were annotated, and only about half of them showed the highest identity to the only other anammox bacterium of which a metagenome assembly had been constructed so far, the freshwater Candidatus Kuenenia stuttgartiensis'. In total, 2016 genes of S.profunda could not be matched to the K.stuttgartiensis metagenome assembly at all, and a similar number of genes in K.stuttgartiensis could not be found in S.profunda. Most of these genes did not have a known function but 98 expressed genes could be attributed to oligopeptide transport, amino acid metabolism, use of organic acids and electron transport. On the basis of the S.profunda metagenome, and environmental metagenome data, we observed pronounced differences in the gene organization and expression of important anammox enzymes, such as hydrazine synthase (HzsAB), nitrite reductase (NirS) and inorganic nitrogen transport proteins. Adaptations of Scalindua to the substrate limitation of the ocean may include highly expressed ammonium, nitrite and oligopeptide transport systems and pathways for the transport, oxidation, and assimilation of small organic compounds that may allow a more versatile lifestyle contributing to the competitive fitness of Scalindua in the marine realm.

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Language(s): eng - English
 Dates: 2012-05-092013-05
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Internal
 Identifiers: eDoc: 675419
ISI: 000318041800004
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Title: Environmental Microbiology
Source Genre: Journal
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Pages: - Volume / Issue: 15 (5 Sp. Iss. SI) Sequence Number: - Start / End Page: 1275 - 1289 Identifier: ISSN: 1462-2912