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  NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis

Matallana-Ramirez, L. P., Rauf, M., Farage-Barhom, S., Dortay, H., Xue, G. P., Droege-Laser, W., et al. (2013). NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis. Molecular Plant, 6(5), 1432-1452. doi:10.1093/mp/sst012.

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Matallana-Ramirez, L. P.1, Autor
Rauf, M.1, Autor
Farage-Barhom, S.1, Autor
Dortay, H.1, Autor
Xue, G. P.1, Autor
Droege-Laser, W.1, Autor
Lers, A.1, Autor
Balazadeh, S.2, Autor           
Mueller-Roeber, B.3, Autor           
Affiliations:
1External Organizations, ou_persistent22              
2Transcription Factors and Gene Regulatory Networks, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753316              
3Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753311              

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 Zusammenfassung: Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 (ANAC092) has recently been shown to play a critical role in positively controlling senescence in Arabidopsis thaliana, however, no direct target gene through which it exerts its molecular function has been identified previously. Here, we report that BIFUNCTIONAL NUCLEASE1 (BFN1), a well-known senescence-enhanced gene, is directly regulated by ORE1. We detected elevated expression of BFN1 already 2 hours after induction of ORE1 in estradiol-inducible ORE1 overexpression lines and 6 hours after transfection of Arabidopsis mesophyll cell protoplasts with a 35S:ORE1 construct. ORE1 and BFN1 expression patterns largely overlap, as shown by promoter - reporter gene (GUS) fusions, while BFN1 expression in senescent leaves and the abscission zones of maturing flower organs was virtually absent in ore1 mutant background. In vitro binding site assays revealed a bipartite ORE1 binding site, similar to the one of ORS1, a paralog of ORE1. A bipartite ORE1 binding site was identified in the BFN1 promoter; mutating the cis element within the context of the full-length BFN1 promoter drastically reduced ORE1-mediated transactivation capacity in transiently transfected Arabidopsis mesophyll cell protoplasts. Furthermore, chromatin-immunoprecipitation (ChIP) demonstrates in vivo binding of ORE1 to the BFN1 promoter. We also demonstrate binding of ORE1 in vivo to the promoters of two other senescence-associated genes, i.e. SAG29/SWEET15 and SINA1, supporting the central role of ORE1 during senescence.

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Sprache(n): eng - English
 Datum: 2013-01-242013
 Publikationsstatus: Erschienen
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 Identifikatoren: Anderer: 23340744
DOI: 10.1093/mp/sst012
ISSN: 1752-9867 (Electronic)1674-2052 (Linking)
URI: http://mplant.oxfordjournals.org/content/early/2013/01/23/mp.sst012.full.pdf
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Titel: Molecular Plant
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Oxford University Press
Seiten: - Band / Heft: 6 (5) Artikelnummer: - Start- / Endseite: 1432 - 1452 Identifikator: Anderer: 1674-2052
CoNE: https://pure.mpg.de/cone/journals/resource/1674-2052