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  A critical review of the N-15(2) tracer method to measure diazotrophic production in pelagic ecosystems

White, A. E., Granger, J., Selden, C., Gradoville, M. R., Potts, L., Bourbonnais, A., et al. (2020). A critical review of the N-15(2) tracer method to measure diazotrophic production in pelagic ecosystems. Limnology and Oceanography: Methods, 18(4), 129-147. doi:10.1002/lom3.10353.

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Mohr20.pdf (Verlagsversion), 2MB
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 Urheber:
White, Angelicque E., Autor
Granger, Julie, Autor
Selden, Corday, Autor
Gradoville, Mary R., Autor
Potts, Lindsey, Autor
Bourbonnais, Annie, Autor
Fulweiler, Robinson W., Autor
Knapp, Angela N., Autor
Mohr, Wiebke1, Autor           
Moisander, Pia H., Autor
Tobias, Craig R., Autor
Caffin, Mathieu, Autor
Wilson, Samuel T., Autor
Benavides, Mar, Autor
Bonnet, Sophie, Autor
Mulholland, Margaret R., Autor
Chang, Bonnie X., Autor
Affiliations:
1Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              

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 Zusammenfassung: Dinitrogen (N-2) fixation is an important source of biologically
reactive nitrogen (N) to the global ocean. The magnitude of this flux,
however, remains uncertain, in part because N-2 fixation rates have been
estimated following divergent protocols and because associated levels of
uncertainty are seldom reported-confounding comparison and extrapolation
of rate measurements. A growing number of reports of relatively low but
potentially significant rates of N-2 fixation in regions such as oxygen
minimum zones, the mesopelagic water column of the tropical and
subtropical oceans, and polar waters further highlights the need for
standardized methodological protocols for measurements of N-2 fixation
rates and for calculations of detection limits and propagated error
terms. To this end, we examine current protocols of the N-15(2) tracer
method used for estimating diazotrophic rates, present results of
experiments testing the validity of specific practices, and describe
established metrics for reporting detection limits. We put forth a set
of recommendations for best practices to estimate N-2 fixation rates
using N-15(2) tracer, with the goal of fostering transparency in
reporting sources of uncertainty in estimates, and to render N-2
fixation rate estimates intercomparable among studies.

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Sprache(n): eng - English
 Datum: 2020-04
 Publikationsstatus: Erschienen
 Seiten: 19
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000526787900001
DOI: 10.1002/lom3.10353
 Art des Abschluß: -

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Titel: Limnology and Oceanography: Methods
  Andere : Limnol. Oceanogr.: Methods
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Waco, Tex. : American Society of Limnology and Oceanography
Seiten: - Band / Heft: 18 (4) Artikelnummer: - Start- / Endseite: 129 - 147 Identifikator: ISSN: 1541-5856
CoNE: https://pure.mpg.de/cone/journals/resource/991042728183802