ausblenden:
Schlagwörter:
-
Zusammenfassung:
The brain cytoplasmic RNA,
BC1
, is a small
non-coding RNA that is found in different RNP par-
ticles, some of which are involved in translational
control. One component of
BC1
-containing RNP
complexes is the fragile X mental retardation
protein (FMRP) that is implicated in translational
repression. Peptide mapping and computational
simulations show that the tudor domain of FMRP
makes specific contacts to
BC1
RNA. Endogenous
BC1
RNA is 2
0
-
O
-methylated in nucleotides that
contact the FMRP interface, and methylation can
affect this interaction. In the cell body
BC1
2
0
-
O
-
methylations are present in both the nucleus and
the cytoplasm, but they are virtually absent at
synapses where the FMRP
–BC1
–mRNA complex
exerts its function. These results strongly suggest
that subcellular region-specific modifications of
BC1
affect the binding to FMRP and the interaction
with its mRNA targets. We finally show that
BC1
RNA has an important role in translation of certain
mRNAs associated to FMRP. All together these
findings provide further insights into the
translational regulation by the FMRP–
BC1
complex
at synapses.
