English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  MYC recruits SPT5 to RNA polymerase II to promote processive transcription elongation.

Baluapuri, A., Hofstetter, J., Dudvarski Stankovic, N., Endres, T., Bhandare, P., Vos, S. M., et al. (2019). MYC recruits SPT5 to RNA polymerase II to promote processive transcription elongation. Molecular Cell, 74(4), 674-687. doi:10.1016/j.molcel.2019.02.031.

Item is

Files

show Files
hide Files
:
3039175.pdf (Publisher version), 7MB
Name:
3039175.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3039175_Suppl_1.pdf (Supplementary material), 6MB
Name:
3039175_Suppl_1.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3039175_Suppl_2.xlsx (Supplementary material), 105KB
Name:
3039175_Suppl_2.xlsx
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3039175_Suppl_3.xlsx (Supplementary material), 80KB
Name:
3039175_Suppl_3.xlsx
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3039175_Suppl_4.pdf (Supplementary material), 12MB
Name:
3039175_Suppl_4.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Baluapuri, A., Author
Hofstetter, J., Author
Dudvarski Stankovic, N., Author
Endres, T., Author
Bhandare, P., Author
Vos, S. M.1, Author           
Adhikari, B., Author
Schwarz, J. D., Author
Narain, A., Author
Vogt, M., Author
Wang, S. Y., Author
Düster, R., Author
Jung, L. A., Author
Vanselow, J. T., Author
Wiegering, A., Author
Geyer, M., Author
Maric, H. M., Author
Gallant, P., Author
Walz, S., Author
Schlosser, A., Author
Cramer, P.1, Author           Eilers, M., AuthorWolf, E., Author more..
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: MYC; RNA polymerase II; SPT5; SPT6; SUPT5H; directionality; elongation rate; processivity; transcription; tumorigenesis
 Abstract: The MYC oncoprotein binds to promoter-proximal regions of virtually all transcribed genes and enhances RNA polymerase II (Pol II) function, but its precise mode of action is poorly understood. Using mass spectrometry of both MYC and Pol II complexes, we show here that MYC controls the assembly of Pol II with a small set of transcription elongation factors that includes SPT5, a subunit of the elongation factor DSIF. MYC directly binds SPT5, recruits SPT5 to promoters, and enables the CDK7-dependent transfer of SPT5 onto Pol II. Consistent with known functions of SPT5, MYC is required for fast and processive transcription elongation. Intriguingly, the high levels of MYC that are expressed in tumors sequester SPT5 into non-functional complexes, thereby decreasing the expression of growth-suppressive genes. Altogether, these results argue that MYC controls the productive assembly of processive Pol II elongation complexes and provide insight into how oncogenic levels of MYC permit uncontrolled cellular growth.

Details

show
hide
Language(s): eng - English
 Dates: 2019-03-272019-05-16
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2019.02.031
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 74 (4) Sequence Number: - Start / End Page: 674 - 687 Identifier: -