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Free keywords:
asymmetric catalysis; combinatorial catalysis; directed evolution; enzyme screening; NMR spectroscopy
Abstract:
Two NMR-based approaches for high-throughput screening of enantioselective catalysts and biocatalysts are described. One version makes use of pseudo-enantiomers or pseudo-meso- compounds based on C-13-labeling. A throughput of at least 1400 ee determinations per day is possible by using an appropriate flow-through cell and an autosampler. The other approach is based on traditional diastereomer formation using a chiral reagent or complexing agent. The ee values are accurate to within +/-2% and +/-5% of the true values.
