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Anion transport; Band 3; Fluorescence microphotolysis; (Erythrocyte membrane)
Abstract:
Fluorescence microphotolysis was used to measure in single resealed human erythrocyte ghosts the band 3-mediated transport of the fluorescent anion N-(7-nitrobenzofurazan-4-yl)-taurine (NBD-taurine). Transport was reduced to less than 5% of the control by the specific inhibitor 4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS). The accuracy of the determination of the rate constant for NBD-taurine influx was approximately ± 15% as calculated from repetitive measurements in individual ghosts. The sample population distribution of the rate constant was slightly skewed towards values larger than the mean. The rate of NBD-taurine transport showed an optimum near pH 7. The Arrhenius plot was linear in the range from 28.5°C to 51°C with an apparent activation enthalphy of 21.4 kcal/mol.
