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  Reconstitution of the light-driven electrogenic ion pump halorhodopsin in black lipid membranes

Bamberg, E., Hegemann, P., & Oesterhelt, D. (1984). Reconstitution of the light-driven electrogenic ion pump halorhodopsin in black lipid membranes. Biochimica et Biophysica Acta-Biomembranes, 773(1), 53-60. doi:10.1016/0005-2736(84)90549-2.

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 Urheber:
Bamberg, Ernst1, Autor           
Hegemann, Peter2, Autor
Oesterhelt, Dieter2, Autor           
Affiliations:
1Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              
2Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Schlagwörter: Halorhodopsin; Black lipid membrane; Photocurrent; Chloride transport Halide transport; (H. halobium)
 Zusammenfassung: Halorhodopsin-containing membrane fragments were isolated from Halobacterium halobium strain L-33, a bacteriorhodopsin-deficient mutant. When these so called Tween-washed membrane fragments are added to one side of a positively charged planar lipid bilayer the system becomes photoelectrically active. Under stationary light conditions photocurrent transients are obtained while the steady-state photocurrent is extremely low. The steady-state photocurrent, however, is considerably increased when the lipid bilayer is doped with the Cl/OH exchanging carrier triphenyltin together with a proton carrier. These results suggest that halorhodopsin is associated with the underlying black lipid membrane in a sandwich-like structure. The photoresponse occurs only in the presence of Cl, Br and I with half-saturation concentrations in the range of 1–10 mM and is virtually independent on the type of the cation (Na+, K+, Mg2+) present. With other anions, such as SO4−2, Fand NO3 no photoresponse was obtained. The results provide direct evidence that halorhodopsin is a light-driven electrogenic pump with a high specificity for halides.

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Sprache(n): eng - English
 Datum: 1984-01-101983-10-052002-12-161984-06-13
 Publikationsstatus: Erschienen
 Seiten: 8
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/0005-2736(84)90549-2
 Art des Abschluß: -

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Titel: Biochimica et Biophysica Acta-Biomembranes
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam : Elsevier
Seiten: - Band / Heft: 773 (1) Artikelnummer: - Start- / Endseite: 53 - 60 Identifikator: ISSN: 0005-2736
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702