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  Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell-like compartments

Jimenez, M., Martos, A., Cabre, E. J., Raso, A., & Rivas, G. (2013). Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell-like compartments. ENVIRONMENTAL MICROBIOLOGY, 15(12, SI), 3158-3168. doi:10.1111/1462-2920.12214.

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Jimenez, Mercedes1, Autor
Martos, Ariadna2, Autor           
Cabre, Elisa J.1, Autor
Raso, Ana2, Autor           
Rivas, German1, Autor
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Schlagwörter: PHYSIOLOGICAL IONIC-STRENGTH; UNILAMELLAR VESICLES; SYNTHETIC BIOLOGY; LIPOSOME MEMBRANES; ARTIFICIAL CELL; LIPID VESICLES; FTSZ RINGS; PROTEIN; RECONSTITUTION; ENCAPSULATION
 Zusammenfassung: The use of artificial lipid membranes, structured as giant unilamellar vesicles (GUVs), provides the opportunity to investigate membrane-associated biological processes under defined experimental conditions. Due to their large size, they are uniquely adapted to investigate the properties and organization (in time and space) of macromolecular complexes incorporated in the vesicle interior by imaging and micro-spectroscopic techniques. Experimental methods to produce giant vesicles and to encapsulate proteins inside them are here reviewed. Previous experimental work to reconstitute elements of the bacterial division machinery in these membrane-like systems is summarized. Future challenges towards reconstructing minimal divisome assemblies in giant vesicles as cytomimetic containers are discussed.

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Sprache(n): eng - English
 Datum: 2013-12
 Publikationsstatus: Erschienen
 Seiten: 11
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000327711100004
DOI: 10.1111/1462-2920.12214
 Art des Abschluß: -

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Titel: ENVIRONMENTAL MICROBIOLOGY
  Alternativer Titel : Environ. Microbiol.
  Andere : Special Issue: Reconstructing the essential bacterial cell cycle machinery.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Seiten: - Band / Heft: 15 (12, SI) Artikelnummer: - Start- / Endseite: 3158 - 3168 Identifikator: ISSN: 1462-2912