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  Video-rate volumetric functional imaging of the brain at synaptic resolution

Lu, R., Sun, W., Liang, Y., Kerlin, A., Bierfeld, J., Seelig, J. D., et al. (2017). Video-rate volumetric functional imaging of the brain at synaptic resolution. Nature Neuroscience. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/28250408.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-D51E-C Version Permalink: https://hdl.handle.net/21.11116/0000-0003-D51F-B
Genre: Journal Article
Alternative Title : Nature Neuroscience

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Lu, Rongwen, Author
Sun, Wenzhi, Author
Liang, Yajie, Author
Kerlin, Aaron, Author
Bierfeld, Jens, Author
Seelig, Johannes D., Author
Wilson, Daniel E.1, Author
Scholl, Benjamin1, Author
Mohar, Boaz, Author
Tanimoto, Masashi, Author
Koyama, Minoru, Author
Fitzpatrick, David1, Author
Orger, Michael B., Author
Ji, Na, Author
Affiliations:
1Kunsthistorisches Institut in Florenz, MPI, Max Planck Society, Via Giuseppe Giusti 44, 50121 Florence, Italy, ou_1254545              

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 Abstract: Neurons and neural networks often extend hundreds of micrometers in three dimensions. Capturing the calcium transients associated with their activity requires volume imaging methods with subsecond temporal resolution. Such speed is a challenge for conventional two-photon laser-scanning microscopy, because it depends on serial focal scanning in 3D and indicators with limited brightness. Here we present an optical module that is easily integrated into standard two-photon laser-scanning microscopes to generate an axially elongated Bessel focus, which when scanned in 2D turns frame rate into volume rate. We demonstrated the power of this approach in enabling discoveries for neurobiology by imaging the calcium dynamics of volumes of neurons and synapses in fruit flies, zebrafish larvae, mice and ferrets in vivo. Calcium signals in objects as small as dendritic spines could be resolved at video rates, provided that the samples were sparsely labeled to limit overlap in their axially projected images.

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 Dates: 2017
 Publication Status: Issued
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 Identifiers: URI: http://www.ncbi.nlm.nih.gov/pubmed/28250408
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Title: Nature Neuroscience
Source Genre: Journal
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