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  Microfluidic tools for quantitative studies of eukaryotic chemotaxis

Beta, C., & Bodenschatz, E. (2011). Microfluidic tools for quantitative studies of eukaryotic chemotaxis. European Journal of Cell Biology, 90, 811-816. doi:10.1016/j.ejcb.2011.05.006.

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 Creators:
Beta, Carsten1, Author              
Bodenschatz, Eberhard1, Author              
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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Free keywords: eukaryotic chemotaxis, Dictyostelium discoideum, microfluidics, caged compounds, numerical simulations
 Abstract: Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.

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Language(s): eng - English
 Dates: 2011-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 575996
DOI: 10.1016/j.ejcb.2011.05.006
 Degree: -

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Title: European Journal of Cell Biology
  Alternative Title : Eur. J. Cell Biol.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 90 Sequence Number: - Start / End Page: 811 - 816 Identifier: ISSN: 0171-9335