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  FCS in STED microscopy: Studying the nanoscale of lipid membrane dynamics.

Müller, V., Honigmann, A., Ringemann, C., Medda, R., Schwarzmann, G., & Eggeling, C. (2013). FCS in STED microscopy: Studying the nanoscale of lipid membrane dynamics. In S. Y. Tetin (Ed.), Fluorescence Fluctuation Spectroscopy (FFS), Part B (pp. 1-38). San Diego, Calif.: Elsevier Acad. Pr.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0013-B020-9 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-CF00-A
Genre: Book Chapter

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 Creators:
Müller, V.1, Author           
Honigmann, A.1, Author           
Ringemann, C.1, Author           
Medda, R.1, Author           
Schwarzmann, G., Author
Eggeling, C.1, Author           
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Details of molecular membrane dynamics in living cells such as lipid protein interactions or the incorporation of molecules into lipid "rafts" are often hidden to the observer because of the limited spatial resolution of conventional far-field optical microscopy. Fortunately, the superior spatial resolution of far-field stimulated-emission-depletion (STED) nanoscopy allows gaining new insights. Applying fluorescence correlation spectroscopy (FCS) in focal spots continuously tuned down to 30 nm in diameter distinguishes free from anomalous molecular diffusion due to transient binding, as for the diffusion of fluorescent phosphoglycero- and sphingolipid analogs in the plasma membrane of living cells. STED-FCS data recorded at different environmental conditions and on different lipid analogs reveal molecular details of the observed nanoscale trapping. Dependencies on the molecular structure of the lipids point to the distinct connectivity of the various lipids to initiate or assist cellular signaling events, but also outline strong differences to the characteristics of liquid-ordered and disordered phase separation in model membranes. STED-FCS is a highly sensitive and exceptional tool to study the membrane organization by introducing a new class of nanoscale biomolecular studies.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Issued
 Pages: L, 325, [18]
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/B978-0-12-405539-1.00001-4
 Degree: -

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Title: Fluorescence Fluctuation Spectroscopy (FFS), Part B
Source Genre: Book
 Creator(s):
Tetin, S. Y., Editor
Affiliations:
-
Publ. Info: San Diego, Calif. : Elsevier Acad. Pr.
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 38 Identifier: ISBN: 978-0-12-405539-1

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Title: Methods in Enzymology
Source Genre: Series
 Creator(s):
Abelson, J. N., Editor
Melvin, I. S., Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: 519 Sequence Number: - Start / End Page: - Identifier: -