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  Cloned duck hepatitis B virus DNA is infectious in Pekin ducks

Sprengel, R., Kuhn, C., Manso, C., & Will, H. (1984). Cloned duck hepatitis B virus DNA is infectious in Pekin ducks. Journal of Virology, 52(3), 932-937. Retrieved from http://jvi.asm.org/content/52/3/932.abstract?maxtoshow%253D%2526HITS%253D10%2526hits%253D10%2526RESULTFORMAT%253D%2526searchid%253D1%2526FIRSTINDEX%253D0%2526volume%253D52%2526firstpage%253D932%2526resourcetype%253DHWCIT.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-D3A1-B Version Permalink: https://hdl.handle.net/21.11116/0000-0000-D3A2-A
Genre: Journal Article

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 Creators:
Sprengel, Rolf1, 2, 3, Author           
Kuhn, Christa, Author
Manso, Constanze, Author
Will, Hans, Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              
3Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society, ou_1497733              

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 Abstract: Approximately 10% of German-bred Pekin ducks were found to be chronically infected with duck hepatitis B virus (DHBV). The genomes of three German DHBV isolates analyzed were closely related but showed substantial restriction site polymorphism compared with U.S. isolates. We tested the infectivity of three sequence variants of cloned DHBV DNA by injecting them into the liver of virus-free ducklings. Most of these animals injected with double-stranded closed-circular or plasmid-integrated dimer DHBV DNA developed viremia, demonstrating the infectivity of all three cloned DHBV DNA variants. The cloned viruses produced were indistinguishable from those from naturally infected animals, implying that our experimental approach can be used to perform a functional analysis of the DHBV genome.

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Language(s): eng - English
 Dates: 1984-07-091984-09-051984-12
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: http://jvi.asm.org/content/52/3/932.abstract?maxtoshow%253D%2526HITS%253D10%2526hits%253D10%2526RESULTFORMAT%253D%2526searchid%253D1%2526FIRSTINDEX%253D0%2526volume%253D52%2526firstpage%253D932%2526resourcetype%253DHWCIT
URI: https://www.ncbi.nlm.nih.gov/pubmed/6492265
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC254616/
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Title: Journal of Virology
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 52 (3) Sequence Number: - Start / End Page: 932 - 937 Identifier: ISSN: 0022-538X
CoNE: https://pure.mpg.de/cone/journals/resource/954925419045