Cell-selective metabolic labeling of proteins

Ngo, J. T.; Champion, J. A.; Mahdavi, A.; Tanrikulu, I. C.; Beatty, K. E.; Connor, R. E.; Yoo, T. H.; Dieterich, D. C.; Schuman, Erin M.; Tirrell, D. A.

doi:10.1038/nchembio.200

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Cell-selective metabolic labeling of proteins

Ngo, J. T., Champion, J. A., Mahdavi, A., Tanrikulu, I. C., Beatty, K. E., Connor, R. E., et al. (2009). Cell-selective metabolic labeling of proteins. Nat Chem Biol, 5(10), 715-7. doi:10.1038/nchembio.200.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-EF30-6 Version Permalink: https://hdl.handle.net/21.11116/0000-0007-EF31-5

Genre: Journal Article

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https://www.ncbi.nlm.nih.gov/pubmed/19668194 (Any fulltext) Open Access status unknown

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Ngo, J. T., Author
Champion, J. A., Author
Mahdavi, A., Author
Tanrikulu, I. C., Author
Beatty, K. E., Author
Connor, R. E., Author
Yoo, T. H., Author
Dieterich, D. C., Author
Schuman, Erin M.¹, Author
Tirrell, D. A., Author

Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710

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Free keywords: Affinity Labels/*metabolism Alanine/analogs & derivatives/metabolism Amino Acyl-tRNA Synthetases/genetics/*metabolism Animals Cell Line Coculture Techniques Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics/metabolism Macrophages, Alveolar/metabolism/microbiology Methionine-tRNA Ligase/genetics/*metabolism Mice Mutagenesis, Site-Directed Mutation Norleucine/analogs & derivatives/metabolism Protein Biosynthesis Proteins/*metabolism

Abstract: Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

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Dates: Date issued: 2009-08-12

Publication Status: Issued

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Identifiers: Other: 19668194
DOI: 10.1038/nchembio.200
ISSN: 1552-4469 (Electronic)1552-4450 (Linking)

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Title: Nat Chem Biol

Source Genre: Journal

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Pages: - Volume / Issue: 5 (10) Sequence Number: - Start / End Page: 715 - 7 Identifier: -