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  Double-flow focused liquid injector for efficient serial femtosecond crystallography

Oberthuer, D., Knoška, J., Wiedorn, M. O., Beyerlein, K. R., Bushnell, D. A., Kovaleva, E. G., et al. (2017). Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7: 44628 (2017). doi:10.1038/srep44628.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-5498-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-5499-0
Genre: Journal Article

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Oberthuer, Dominik1, Author
Knoška, Juraj1, 2, Author
Wiedorn, Max O.1, 2, Author
Beyerlein, Kenneth R.1, Author
Bushnell, David A.3, Author
Kovaleva, Elena G.4, Author
Heymann, Michael1, Author
Gumprecht, Lars1, Author
Kirian, Richard A.5, Author
Barty, Anton1, Author
Mariani, Valerio1, Author
Tolstikova, Aleksandra1, 2, Author
Adriano, Luigi6, Author
Awel, Salah1, 7, Author
Barthelmess, Miriam1, Author
Dörner, Katerina1, Author
Paulraj, Lourdu Xavier1, 2, 8, Author           
Yefanov, Oleksandr1, Author
James, Daniel R.5, Author
Nelson, Garrett5, Author
Wang, Dingjie5, AuthorCalvey, George9, AuthorChen, Yujie9, AuthorSchmidt, Andrea10, AuthorSzczepek, Michael10, AuthorFrielingsdorf, Stefan11, AuthorLenz, Oliver11, AuthorSnell, Edward12, AuthorRobinson, Philip J.4, AuthorŠarler, Božidar13, 14, AuthorBelšak, Grega14, AuthorMaček, Marjan14, AuthorWilde, Fabian15, AuthorAquila, Andrew16, AuthorBoutet, Sébastien16, AuthorLiang, Mengning16, AuthorHunter, Mark S.16, AuthorScheerer, Patrick10, AuthorLipscomb, John D.17, AuthorWeierstall, Uwe5, AuthorKornberg, Roger D.3, AuthorSpence, John C. H.5, AuthorPollack, Lois9, AuthorChapman, Henry N.1, 2, 17, AuthorBajt, Saša6, Author more..
Affiliations:
1Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany, ou_persistent22              
2Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              
3Department of Structural Biology, Stanford University, School of Medicine, Stanford, California 94305, USA, ou_persistent22              
4SSRL, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA, ou_persistent22              
5Department of Physics, Arizona State University, Tempe, AZ, USA, ou_persistent22              
6Photon Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607 Hamburg, Germany, ou_persistent22              
7Centre for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              
8International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
9School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA, ou_persistent22              
10Institut für Medizinische Physik und Biophysik, Group Protein X-ray Crystallography and Signal Transduction, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany, ou_persistent22              
11Institut für Chemie, Sekr. PC14, Technische Universität Berlin, Straße des 17, Juni 135, 10623 Berlin, Germany, ou_persistent22              
12Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA, ou_persistent22              
13Laboratory for Multiphase Processes, University of Nova Gorica, Vipavska 13, SI-5000 Nova Gorica, Slovenia, ou_persistent22              
14Laboratory for Simulation of Materials and Processes, Institute of Metals and Technology, Lepi pot 11, SI-1000 Ljubljana, Slovenia, ou_persistent22              
15Helmholtz-Zentrum Geesthacht, Max-Planck-Straße 1, 21502 Geesthacht, Germany, ou_persistent22              
16LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA, ou_persistent22              
17Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA, ou_persistent22              

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Free keywords: Fluid dynamics, Nanocrystallography
 Abstract: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

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Language(s): eng - English
 Dates: 2017-01-062017-02-102017-03-16
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/srep44628
 Degree: -

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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 44628 (2017) Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322